产品名称
SIGMAFAST™ 对硝基苯基磷酸酯片, tablet, To prepare 5 mL
Quality Level
form
tablet
solubility
deionized water: soluble 5 mL
storage temp.
−20°C
Application
SIGMA FAST p-磷酸硝基苯酯片剂用于：
- 定量非分泌型碱性磷酸酶，监测成骨样细胞的分化情况（SaoS-2）
- 染色小鼠前成骨细胞 MC3T3-E1，进行碱性磷酸酶测定
- 用作 ExtrAvidin 碱性磷酸酶的酶作用物®，通过酶联免疫吸附法（ELISA）测定血红蛋白结合α-突触核蛋白
- 用作碱性磷酸酶偶联抗体的酶作用物，进行Abirin毒素的ELISA检测
开发用作可溶性底物，用于检测酶免疫分析法（EIA）中碱性磷酸酶的活性。 pNPP是高灵敏度碱性磷酸酶EIA检测的首选底物。将一片pNPP和一片Tris缓冲片溶解在去离子水中即得即用型底物。 SIGMAFAST pNPP每片可产生含有1.0 mg/ml pNPP、0.2 M Tris缓冲液的溶液。使用pNPP的EIA应用可以在定时实验中读数，或用3 N NaOH终止反应后延迟读数。SIGMAFAST™ 仅需要添加蒸馏水或去离子水即可制备活性底物溶液。片剂用箔包单个包装。
Preparation Note
将一组片剂溶于5 mL去离子水中，即可得到含有pNPP的即用型缓冲液。
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Conall H Watson et al.
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Ruklanthi de Alwis et al.
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Lan Xu et al.
Experimental cell research, 360(2), 413-420 (2017-09-30)
The highly glycosylated bone sialoprotein (BSP) is an abundant non-collagenous phosphoprotein in bone which enhances osteoblast differentiation and new bone deposition in vitro and in vivo. However, the structural details of its different glycosylation linkages have not been well studied
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N1891-50SET | 04061835557943 |
| N1891-5SET | 04061834109907 |