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N3910

中和液 B

Name: 中和液 B
Brand: SIGMA

别名:

DNA neutralization solution, neutralization buffer DNA extraction

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关于此项目

NACRES:

NA.25

UNSPSC Code:

12161703

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属性

grade

Molecular Biology

Quality Level

200

form

liquid

feature

Direct PCR

technique(s)

DNA amplification: suitable, DNA extraction: suitable, PCR: suitable

input

crude DNA

storage temp.

−20°C

说明

General description

这种中和缓冲液B要与 Sigma′REDExtract-N-Amp^™ 组织 PCR.试剂盒一起使用。该缓冲液用于中和DNA提取液，并与REDExtract-N-Amp PCR 反应混合液及PCR引物一起用于扩增靶标DNA。 REDExtract-N-Amp试剂盒包括足够体积的中和缓冲液B，每次提取至少可中和一次。不过，如果需要更多的扩增反应，则可能需要补充额外的中和缓冲液。

Application

中和缓冲液B 已用于改良 Sigma Extract-N-Amp^™提取程序。它还用于小鼠的基因分型，在聚合酶链反应 (PCR) 之前可使用Sigma的REDExtract-N-Amp^™ PCR 反应混合物来中和DNA提取液。

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

pictograms

GHS07,GHS09

signalword

Warning

hcodes

H315,H317,H319,H411

pcodes

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

法规信息

低风险生物材料

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分析证书(COA)

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实验方案

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish

Varshney GK, et al.

Nature Protocols, 11(12), 2357-2357 (2016)

Neurogenesis decreases during brain maturation from adolescence to adulthood.

Jun He et al.

Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)

Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we

Activated K-ras and INK4a/Arf deficiency cooperate during the development of pancreatic cancer by activation of Notch and NF-κB signaling pathways.

Zhiwei Wang et al.

PloS one, 6(6), e20537-e20537 (2011-06-16)

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer-related death in the United States, suggesting that novel strategies for the prevention and treatment of PDAC are urgently needed. K-ras mutations are observed in >90% of pancreatic cancer, suggesting

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