usage
sufficient for 350 purifications
Quality Level
technique(s)
DNA purification: suitable
storage temp.
15-25°C, room temp
General description
GenElute血液基因组DNA试剂盒可以简单、方便地从新鲜或长时间存放（超过24小时）的全血中分离高纯度基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。
Application
纯化所得的基因组DNA可用于以下下游应用：
- 限制性内切酶酶切
- PCR
- Southern印迹
- 测序反应
- 克隆
Biochem/physiol Actions
起始材料首先溶解在含有离液盐的溶液之中，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。随附一份Prewash Solution，可帮助与全血样本长时间存放（超过24小时）相关的污染物。在洗涤去除污染物后，将DNA放在200 mL的Tris-EDTA溶液中洗脱。
预期基因组DNA得率取决于所用的起始材料的数量和性质（例如，从200 μl 新鲜的全血之中，可在一小时内分离出4至10 μg RNase A处理的DNA）。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。
预期基因组DNA得率取决于所用的起始材料的数量和性质（例如，从200 μl 新鲜的全血之中，可在一小时内分离出4至10 μg RNase A处理的DNA）。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。
Features and Benefits
- 起始材料：不超过200 μl新鲜或长时间存放的血液
- 预期得率：至多10 μg
- 洗脱体积：400 μl
- 所需时间：<40分钟
- A260/A280比率：1.6 - 1.9
- 兼容多种抗凝血素、包括EDTA、肝素和柠檬酸钠
- 通常得率为4-10 μg/200 μL全血样本
- 可获得A260/A280 比率介于1.6-1.9之间的高纯度基因组DNA
- 可分离长度不超过50 kb的DNA
Other Notes
更多信息，请见www.sigma-aldrich.com/genomicdna。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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signalword
Danger
target_organs
Respiratory system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
存储类别
10 - Combustible liquids
法规信息
低风险生物材料
常规特殊物品
此项目有
实验方案
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NA2020-1KT | 04061835573738 |