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Merck
CN

P3397

磷酸葡萄糖变位酶 来源于兔肌肉

ammonium sulfate suspension, ≥100 units/mg protein

别名:

PGM, α-D-葡萄糖-1,6-二磷酸酶, α-D-葡萄糖磷酸转移酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
Biological source:
rabbit muscle
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biological source

rabbit muscle

Quality Level

description

ammonium sulfate suspension, >=100 units/mg protein

form

ammonium sulfate suspension

specific activity

≥100 units/mg protein

storage condition

(Tightly closed)

technique(s)

activity assay: suitable

color

white to light yellow

foreign activity

lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05%

shipped in

wet ice

storage temp.

2-8°C

General description

适合研究领域:细胞信号传导

磷酸葡萄糖变位酶(PGM),是一种保守酶,在动物、植物和微生物中大量存在,几乎分布于所有组织中。

Application

本品(磷酸葡萄糖变位酶)已用于:

  • 研究人类的糖原合成与磷酸葡萄糖变位酶缺乏
  • 通过检测磷酸葡萄糖变位酶的活性研究代谢调控
  • 试验A(参见文献),检测BL21(DE3)C43转化细胞中的糖原代谢密切相关酶
  • 蔗糖合酶(SuSy)和ADP葡萄糖焦磷酸化酶(AGPase)检测(3)
  • 在酶活性测定中结合烟酰胺腺嘌呤二核苷酸磷酸(NADP+)还原反应,测定来自蔗糖和无机磷酸(Pi)的葡萄糖-1-磷酸

Biochem/physiol Actions

磷酸葡萄糖变位酶(PGM)在糖酵解和糖异生中起着至关重要的作用。参与糖原和昆虫海藻糖的代谢。磷酸葡萄糖变位酶也参与植物和某些微生物的发育。葡萄糖-6-磷酸是核心代谢产物,因此PGM是蛋白质、脂质和核酸的代谢关键,对植物发育至关重要。磷酸葡萄糖变位酶催化葡萄糖-6-磷酸(G-6-P)和葡萄糖-1-磷酸(G-1-P)的相互转变。

Physical form

(NH4)2SO4,pH 6.0,含 0.01%EDTA 的晶体混悬液

Analysis Note

缩二脲法测定蛋白

Other Notes

1 个单位将在 30°C、pH 7.4 下每分钟将 1.0 μmolα-D-葡萄糖 1-磷酸转化为 α-D-葡萄糖 6-磷酸。


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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

低风险生物材料

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

以硫酸铵悬浮液形式提供的酶的使用指南

Instructions for working with enzymes supplied as ammonium sulfate suspensions

相关内容


Phosphoglucomutase; mechanism of action.
V JAGANNATHAN et al.
The Journal of biological chemistry, 179(2), 569-575 (1949-06-01)
M B Dworkin et al.
The Journal of biological chemistry, 262(35), 17038-17045 (1987-12-15)
When 32P-labeled phosphoenolpyruvate is injected into Xenopus laevis oocytes, a 50-60-kDa protein of subunit size Mr 29,000 is rapidly labeled, followed by a second (monomeric) protein of 66 kDa concomitant with the loss of label from the first protein. We
Asbjørg Stray-Pedersen et al.
American journal of human genetics, 95(1), 96-107 (2014-06-17)
Human phosphoglucomutase 3 (PGM3) catalyzes the conversion of N-acetyl-glucosamine (GlcNAc)-6-phosphate into GlcNAc-1-phosphate during the synthesis of uridine diphosphate (UDP)-GlcNAc, a sugar nucleotide critical to multiple glycosylation pathways. We identified three unrelated children with recurrent infections, congenital leukopenia including neutropenia, B



全球贸易项目编号

货号GTIN
P3397-500UN04061834367833
P3397-1KU04061833493106
P3397-2.5KU04061833493113
P3397-200UN04061834367826