biological source
rabbit muscle
Quality Level
description
ammonium sulfate suspension, >=100 units/mg protein
form
ammonium sulfate suspension
specific activity
≥100 units/mg protein
storage condition
(Tightly closed)
technique(s)
activity assay: suitable
color
white to light yellow
foreign activity
lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05%
shipped in
wet ice
storage temp.
2-8°C
General description
适合研究领域:细胞信号传导
磷酸葡萄糖变位酶(PGM),是一种保守酶,在动物、植物和微生物中大量存在,几乎分布于所有组织中。
磷酸葡萄糖变位酶(PGM),是一种保守酶,在动物、植物和微生物中大量存在,几乎分布于所有组织中。
Application
本品(磷酸葡萄糖变位酶)已用于:
- 研究人类的糖原合成与磷酸葡萄糖变位酶缺乏
- 通过检测磷酸葡萄糖变位酶的活性研究代谢调控
- 试验A(参见文献),检测BL21(DE3)C43转化细胞中的糖原代谢密切相关酶
- 蔗糖合酶(SuSy)和ADP葡萄糖焦磷酸化酶(AGPase)检测(3)
- 在酶活性测定中结合烟酰胺腺嘌呤二核苷酸磷酸(NADP+)还原反应,测定来自蔗糖和无机磷酸(Pi)的葡萄糖-1-磷酸
Biochem/physiol Actions
磷酸葡萄糖变位酶(PGM)在糖酵解和糖异生中起着至关重要的作用。参与糖原和昆虫海藻糖的代谢。磷酸葡萄糖变位酶也参与植物和某些微生物的发育。葡萄糖-6-磷酸是核心代谢产物,因此PGM是蛋白质、脂质和核酸的代谢关键,对植物发育至关重要。磷酸葡萄糖变位酶催化葡萄糖-6-磷酸(G-6-P)和葡萄糖-1-磷酸(G-1-P)的相互转变。
Physical form
(NH4)2SO4,pH 6.0,含 0.01%EDTA 的晶体混悬液
Analysis Note
缩二脲法测定蛋白
Other Notes
1 个单位将在 30°C、pH 7.4 下每分钟将 1.0 μmolα-D-葡萄糖 1-磷酸转化为 α-D-葡萄糖 6-磷酸。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
此项目有
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Phosphoglucomutase; mechanism of action.
V JAGANNATHAN et al.
The Journal of biological chemistry, 179(2), 569-575 (1949-06-01)
M B Dworkin et al.
The Journal of biological chemistry, 262(35), 17038-17045 (1987-12-15)
When 32P-labeled phosphoenolpyruvate is injected into Xenopus laevis oocytes, a 50-60-kDa protein of subunit size Mr 29,000 is rapidly labeled, followed by a second (monomeric) protein of 66 kDa concomitant with the loss of label from the first protein. We
Asbjørg Stray-Pedersen et al.
American journal of human genetics, 95(1), 96-107 (2014-06-17)
Human phosphoglucomutase 3 (PGM3) catalyzes the conversion of N-acetyl-glucosamine (GlcNAc)-6-phosphate into GlcNAc-1-phosphate during the synthesis of uridine diphosphate (UDP)-GlcNAc, a sugar nucleotide critical to multiple glycosylation pathways. We identified three unrelated children with recurrent infections, congenital leukopenia including neutropenia, B
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P3397-500UN | 04061834367833 |
| P3397-1KU | 04061833493106 |
| P3397-2.5KU | 04061833493113 |
| P3397-200UN | 04061834367826 |