P4978
磷酸酶(碱性) 来源于小牛肠
buffered aqueous glycerol solution
别名:
CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.53
MDL number:
Concentration:
≥10,000 units/mL
grade
Molecular Biology
form
buffered aqueous glycerol solution
mol wt
~80 kDa
concentration
≥10,000 units/mL
UniProt accession no.
foreign activity
DNase, RNase, none detected
shipped in
wet ice
storage temp.
−20°C
Gene Information
cow ... ALPI(280993)
General description
碱性磷酸酶是一种磷酸单酯酶，可催化核酸的末端磷酸盐裂解。游离 5′-OH 可用多核苷酸激酶和 γ32P-ATP 磷酸化，以产生 γ32P 末端标记核酸。通过碱性磷酸酶去磷酸化可以防止线性化的克隆载体再环化。
Application
通常用于在分子克隆反应过程中从核酸中去除5'-末端磷酸盐。
Other Notes
5 mM EDTA (pH 8) 中的碱性磷酸酶将在 75℃ 下不可逆地加热灭活 10 分。
一个单位将在37℃ 下每分钟水解 1.0μ摩尔对 -硝基苯磷酸盐。
碱性磷酸酶以50% (w/v) 甘油溶液的形式提供，溶液中还含有 10 mM Tris-HCl (pH 8.2)、50 mM KCl、1 mM MgCl 2 和 0.1 mM ZnCl 2 。还包括 10X CIP 缓冲液（产品编号 C3225）。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
实验方案
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
CIP可用于脱去DNA、RNA及核糖和脱氧核糖核苷三磷酸上的5’-磷酸基团。如何进行DNA去磷酸化的详细实验方案。
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
Jong Kil Lee et al.
The Journal of experimental medicine, 211(8), 1551-1570 (2014-07-23)
In Alzheimer's disease (AD), abnormal sphingolipid metabolism has been reported, although the pathogenic consequences of these changes have not been fully characterized. We show that acid sphingomyelinase (ASM) is increased in fibroblasts, brain, and/or plasma from patients with AD and
Jean-Paul Lallès
Nutrition reviews, 72(2), 82-94 (2014-02-11)
Important protective roles of intestinal alkaline phosphatase (IAP)--including regulation of intestinal surface pH, absorption of lipids, detoxification of free nucleotides and bacterial lipopolysaccharide, attenuation of intestinal inflammation, and possible modulation of the gut microbiota--have been reviewed recently. IAP is modulated
Yixing Cheng et al.
Journal of biomedical nanotechnology, 10(2), 287-298 (2014-04-18)
Scaffold architecture, surface topography, biochemical and mechanical cues have been shown to significantly improve cellular events and in vivo tissue regeneration. Specifically electrospun nanofiber matrices have gained tremendous interest due to their intrinsic structural resemblance to native tissue extracellular matrix
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P4978-500UN | 04061834375586 |
| P4978-1KU | 04061835574667 |