P8811
蛋白酶 来源于灰色链霉菌
powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
别名:
Actinase E, Pronase E
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352207
EC Number:
232-909-5
NACRES:
NA.75
eCl@ss:
32160410
MDL number:
Form:
powder
product line
BioReagent
Quality Level
form
powder
specific activity
≥3.5 units/mg solid
technique(s)
cell culture | embryo: suitable
impurities
starch, essentially free
storage temp.
−20°C
General description
通过在80 °C以上加热15-20分钟以完全灭活。
Application
蛋白酶通常用于核酸的分离过程，通过补充0.2％十二烷基硫酸钠和10 mM EDTA并孵育0.5-3.0小时。
该酶比已知碱性蛋白酶在更高的pH范围内更具活性，即使在0.2N NaOH溶液中也显示出了蛋白水解活性。该酶用于不溶性蛋白的蛋白水解和蛋白的结构研究。
Biochem/physiol Actions
一种具有包括细胞外丝氨酸蛋白酶在内至少三种蛋白水解活性的混合物。通常，丝氨酸蛋白酶可显示出广泛的底物特异性，而这被视为通过分子中由一个Asp、一个His和Ser残基组成的活性位点所介导的。该酶更倾向于水解谷氨酸或天冬氨酸的羧基侧的肽键。
Preparation Note
收集自灰色链霉菌的培养基肉汤中。
Analysis Note
含有约 25％ 的醋酸钙。
Other Notes
一种不常见的非特异性蛋白酶。
在 pH7.5，37℃ 下，一个单元每分钟将水解酪蛋白，并产生相当于 1.0 μmole（181 μg）酪氨酸的颜色（使用 Folin-Ciocalteu 试剂显色）。
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
商品
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
Laura D Mathies et al.
BMC genomics, 20(1), 427-427 (2019-05-30)
The two Caenorhabditis elegans somatic gonadal precursors (SGPs) are multipotent progenitors that generate all somatic tissues of the adult reproductive system. The sister cells of the SGPs are two head mesodermal cells (hmcs); one hmc dies by programmed cell death
P C Chang et al.
Gene, 88(1), 87-95 (1990-03-30)
The gene (npr) encoding an extracellular neutral metalloprotease (Npr) from Streptomyces cacaoi YM15 was cloned in Streptomyces lividans using pIJ702 as a vector. The nucleotide (nt) sequence of npr was determined. The deduced open reading frame encoded 550 amino acids
S Blumberg et al.
European journal of biochemistry, 136(1), 151-154 (1983-10-17)
A series of 2-mercaptoacetyl-dipeptides, a potential group of metalloendopeptidase inhibitors, has been synthesized by coupling the N-hydroxysuccinimide ester of S-acetyl-2-mercaptoacetic acid with hydrophobic dipeptide methyl ester hydrochlorides, followed by hydrolysis with NaOH in aqueous methanol and acidification with HCl. Thus
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P8811-1G | 04061834401834 |
| P8811-100MG | 04061835569311 |
| P8811-5G | 04061835507368 |