Quality Level
form
liquid
usage
sufficient for 50 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s PCR 50-2,000 bp标记包含8个dsDNA片段,间隔50-2,000个碱基,间隔均匀。 可以将5μl梯子直接装在琼脂糖或聚丙烯酰胺凝胶上的单个泳道中。
Application
PCR 50 - 2,000 bp标记物可用作DNA凝胶电泳的标记物。
Other Notes
Sigma′s PCR 50 - 2,000 bp标记物为两个试管:
- 1瓶(250 μl体积)PCR标记物
- 1瓶6×上样缓冲液(250 μl体积),用于稀释样品
为获得最佳分离度,推荐琼脂糖凝胶浓度为 2.0%。
在可预测的间隔(阶梯)双链DNA片段中包含8个条带,50-2,000 bp。
存储类别
10 - Combustible liquids
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
实验方案
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
商品
选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Undergraduate virology exercises demonstrate conventional and real-time PCR using commercially available HIV primers and noninfectious target.
Sulzinski MA, et al.
Biochemistry and Molecular Biology Education, 37, 232-235 (2009)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P9577-1VL | 04061835573882 |