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Merck
CN

PF0100

蛋白酶检测试剂盒(荧光法)

high sensitivity assay

别名:

蛋白酶活性分析试剂盒

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关于此项目

NACRES:
NA.26
UNSPSC Code:
12352202
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Quality Level

shipped in

dry ice

storage temp.

−20°C

Application

蛋白酶荧光检测试剂盒已用于:
  • 通过基于酪蛋白的蛋白酶测定,检测蛋白水解活性
  • 检测真菌菌株的细胞外蛋白水解活性
  • 定量线粒体蛋白酶活性
  • 测定土壤溶液中的蛋白酶生成

检测限:最大50ng胰蛋白酶

Preparation Note

提供了足以用于200次一毫升反应体系的试剂。

三氯乙酸(TCA)组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。

Analysis Note

已测试过其在全部4种蛋白酶水解类别中的检测效果(丝氨酸、天冬氨酸、半胱氨酸和金属蛋白酶)。


仅试剂盒组分

产品编号
说明

  • Incubation buffer 25 mL

  • FITC-casein solution 5 mL

  • Assay buffer 200 mL

  • Protease standard (trypsin) 20 μg

  • FITC control 50 mg

  • Trichloroacetic acid (TCA) solution 30 mL

signalword

Danger

target_organs

Respiratory system

存储类别

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1 - Skin Sens. 1 - STOT SE 3

法规信息

低风险生物材料

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

商品

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.


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Selena Gimenez-Ibanez et al.
PLoS biology, 12(2), e1001792-e1001792 (2014-02-22)
Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes a suite of virulence effector proteins into the host cytoplasm, and the production of a number of toxins such as coronatine (COR), which is a mimic
M Lee et al.
Neuroscience, 229, 164-175 (2012-10-27)
Astrocytes become activated in degenerative neurological diseases. In order to gain a greater understanding of the inflammatory factors released upon activation, we stimulated adult human astrocytes with interferon-gamma and examined the resultant conditioned medium (CM) for toxicity against differentiated human



全球贸易项目编号

货号GTIN
PF0100-1KT04061834413271