recombinant
expressed in E. coli
Quality Level
grade
Molecular Biology
form
buffered aqueous solution
mol wt
~50 kDa
concentration
20-40 units/μL
shipped in
dry ice
storage temp.
−20°C
General description
核糖核酸酶抑制剂通过形成紧密非共价的1:1复合物抑制RNase活性。 它来源于大肠杆菌，大肠杆菌表达人胎盘核糖核酸酶的部分结构。 抑制RNA酶A、B和C。不抑制RNA酶H、1、T1、S1核酸酶、SP6、T7 RNA聚合酶、T3 RNA聚合酶、AMV逆转录酶、M-MLV逆转录酶或Taq聚合酶。 该抑制剂可通过苯酚萃取或在65°C 下加热10分钟去除。抑制的pH范围是 pH 5.5–9 （pH 7–8之间抑制能力最高）。
Application
核糖核酸酶抑制剂适用于核糖核酸酶的体外 抑制，包括以下应用如：
- 从mRNA合成cDNA
- 逆转录聚合酶链反应（RT-PCR）
- 体外 转录和翻译
- mRNA 降解的保护
- 甲醛交联lncRNA 纯化
- RNA荧光原位杂交（FISH）
适于体外抑制核糖核酸酶，包括cDNA合成、RT-PCR以及体外转录和翻译等步骤。
Biochem/physiol Actions
通过形成紧密非共价的 1:1 复合物抑制 RNase。
Physical form
溶于 20 mM HEPES-KOH (pH 7.6)、50 mM KCl、8 mM DTT 和 50% 甘油中。
Other Notes
- 应当避免变性条件（即脲或温度≥50 °C），因为它们会导致活性核糖核酸酶从复合物上释放。
- 该产品仅用于研发，不用于药物、家庭或其他用途。
一个单位的抑制剂可将一个胞苷2′:3′-环单磷酸盐体系中5ng核糖核酸酶A的活性降低50%。
核糖核酸酶抑制剂，含20 mM HEPES-KOH(pH 7.6)、50 mM KCl、8 mM DTT和50%甘油。
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R1158-2.5KU | 04061836694203 |
| R1158-10KU | 04061833255575 |