Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
洛斯维帕克纪念研究所培养基通常被称为PRMI。 是一种用于细胞培养和组织培养的培养基。该培养基含有磷酸，适用于含有5%二氧化碳的气体。它可用于人淋巴细胞生长。
Application
RPMI-1640培养基用于以下应用：
- 用于细胞培养基（即在补充其它成分的 RPMI 1640 培养基中培养鼠胸腺瘤 EL-4 细胞）
- 用于脾细胞的分枝杆菌感染（在不含抗生素的 RPMI 1640 培养基中）
- 用于细胞分离和培养
- 用于淋巴细胞分离和培养、ELISPOT分析、体外增殖检测的培养基
- 用于gp39 基因表达和 CD40-免疫球蛋白结合检测的培养基
- 用于制备抗真菌剂
Preparation Note
按照每升培养基10.4克粉末配制。
补充2.0 g/L碳酸氢钠。
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
Hui Tian et al.
Analytical chemistry, 88(23), 11384-11389 (2016-11-02)
MicroRNA (miRNA) analysis in a single cell is extremely important because it allows deep understanding of the exact correlation between the miRNAs and cell functions. Herein, we wish to report a highly sensitive and precisely quantitative assay for miRNA detection
M Farrington et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(3), 1099-1103 (1994-02-01)
Common variable immunodeficiency (CVI) is characterized by hypogammaglobulinemia and recurrent bacterial infections due to failure of CVI B cells to differentiate in vivo into immunoglobulin-secreting plasma cells. We hypothesized that T-cell dysfunction resulting in abnormal contact-mediated B-cell activation may play
T P Szatrowski et al.
Cancer research, 51(3), 794-798 (1991-02-01)
Few nonphagocytic cells are known to generate reactive oxygen intermediates. Based on horseradish peroxidase-dependent, catalase-inhibitable oxidation of fluorescent scopoletin, seven human tumor cell lines constitutively elaborated H2O2 at rates (up to 0.5 nmol/10(4) cells/h) large enough that cumulative amounts at
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R6504-1L | 04061837464911 |
| R6504-963L | 04061837464935 |
| R6504-1000L | 04061837464898 |
| R6504-100L | 04061837464904 |
| R6504-10L | 04061836695866 |
| R6504-10X1L | 04061835569328 |
| R6504-50L | 04061836695873 |