产品名称
碳酸氢钠 溶液, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
sterility
sterile-filtered
product line
BioReagent
form
solution (7.5%)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.8-8.2
storage temp.
2-8°C
SMILES string
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
General description
碳酸氢钠(NaHCo3)是实验室常用的碱性缓冲剂(5)。加入细胞培养基后，配合培养箱中的二氧化碳共同维持适宜的pH。除了缓冲作用，碳酸氢钠还能作为钠源，在发酵时用于合成葡糖酸钠。
Application
碳酸氢钠溶液可用于：
- 作为Hanks′平衡盐溶液(HBSS)和recording medium组分，用于大鼠或小鼠组织外植体的培养
- 作为Dulbecco′改良Eagle′培养基(DMEM)组分，用于小鼠组织外植体的培养
- 作为最低必需培养基(MEM)组分，用于研究对人结直肠腺癌细胞和乳腺腺癌的细胞毒性
- 制备胶原混合物液滴，用于3D胶原出芽实验
- 制备胶原基质，用于大鼠表皮角质形成细胞(REK)器官型培养
Preparation Note
用细胞培养级水配制。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
3-D Culture Matrix™胶原I可单独或与其他基底膜成分一起用作在其上生长细胞的凝胶或培养基添加剂，用于研究体外三维的细胞生长和分化。
3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.
Nguyen T K Vo et al.
Developmental and comparative immunology, 92, 140-149 (2018-11-20)
Viral double-stranded (ds)RNA is a potent pathogen-associated molecular pattern (PAMP), capable of inducing a strong antiviral state within the cell, protecting the cell from virus infection. In mammals and fish, sensing extracellular dsRNA is mediated by cell-surface class A scavenger
Mitofusin 2 regulates neutrophil adhesive migration and the actin cytoskeleton.
Zhou, et al.
Journal of Cell Science, 133 (2021)
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S8761-100ML | 04061838260031 |
| S8761-500ML | 04061836961046 |