SEQXE
SeqPlex DNA扩增试剂盒
For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.
别名:
SeqPlex增强型DNA扩增试剂盒, WGA 试剂盒, 全基因组扩增试剂盒
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关于此项目
NACRES:
NA.55
UNSPSC Code:
41121800
Quality Level
technique(s)
whole genome amplification: suitable
dilution
(WGA)
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
General description
面向全基因组扩增（WGA）的 SeqPlex™ Enhanced DNA 扩增试剂盒专用于对极小量或降解/高度片段化的 DNA 进行下一代测序（NGS）。 染色质免疫沉淀（ChIP）或福尔马林固定的石蜡包埋组织样品（FFPE）的DNA含量往往无法满足成功制备下一代测序文库的需求。通过 SeqPlex 试剂盒可对这类 DNA 以及其他用于 NGS 的小量/高度片段化 DNA 样品进行预扩增。 该试剂盒是 WGA 产品线的延伸，旨在集成到 Illumina®、SOLiD™ 或 454 测序工作流程中。
Application
SeqPlex™ DNA 扩增试剂盒已用于全基因组扩增。
Features and Benefits
- 随机引物技术可扩增片段化的 DNA，如 ChIP 或 FFPE
- 便于从低至 100 pg 的 ChIP DNA 进行测序
- 增强引物，可实现完整的基因组覆盖、最小的序列偏差、引物去除，扩增子大小非常适合新一代测序
- 与Illumina®、SOLiD™或454文库制备兼容，用于新一代测序
Other Notes
SEQXE-500RXN 是按需生产。获取更多信息，请发邮件至techserv@sial.com 联系技术服务。
Legal Information
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书 & 价格
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
法规信息
常规特殊物品
此项目有
实验方案
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
Sigma Imprint染色质免疫沉淀试剂盒利用检测板系统，可实现快速的高通量ChIP检测
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Optimizing sparse sequencing of single cells for highly multiplex copy number profiling.
Baslan T, et al.
Genome Research, 25(5), 714-724 (2015)
SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.
Mian S A, et al.
Nature Communications, 6, 10004-10004 (2015)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SEQXE-10RXN | 04061837018138 |
| SEQXE-500RXN | 04061837018145 |
| SEQXE-50RXN | 04061837018176 |