product line
MISSION®
shelf life
≥2 yr at ~4 °C
storage temp.
2-8°C
General description
To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols
www.sigmaaldrich.com/mission-protocols
Application
MISSION® ExpressMag® Super Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product can be used in conjunction with standard sized well plates, T-25 through T-75 tissue culture flasks, and up to 5 cm dishes. The kit has been documented to enhance MISSION® lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag™ system.
Legal Information
ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Do not autoclave! Clean surface of magnet with 70% isopropyl alcohol.
Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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实验方案
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
商品
获取处理慢病毒、优化实验设置、测定慢病毒颗粒滴度以及选择实用转导产品方面的贴士。
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SHM01-1KT | 04061826727218 |