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SML2135

生物素基酪酰胺

Name: 生物素基酪酰胺
Brand: SIGMA

≥97% (HPLC), powder, tyramide signal amplifier

别名:

生物素酚, （3AS，4S，6AR）-六氢-N- [2-（4-羟基苯基）乙基] -2-氧代-1H-噻吩并[3，4-d]咪唑-4-戊

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关于此项目

经验公式（希尔记法）:

C₁₈H₂₅N₃O₃S

化学文摘社编号:

41994-02-9

分子量:

363.47

UNSPSC Code:

12352200

NACRES:

NA.77

MDL number:

MFCD27953026

Assay:

≥97% (HPLC)

Form:

powder

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属性

产品名称

生物素基酪酰胺, ≥97% (HPLC)

assay

≥97% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 2 mg/mL, clear

storage temp.

−20°C

SMILES string

O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O

InChI

1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1

InChI key

VZWXNOBHWODXCW-ZOBUZTSGSA-N

说明

Application

生物素酪胺或生物素-苯酚已用于邻近标记法蛋白质鉴定。

Biochem/physiol Actions

生物素酪胺是一种酪胺信号放大试剂，适于免疫组织化学(IHC)和原位杂交实验，以及显色和荧光检测。探针初步结合之后，接着用 HRP 标记的抗体或链霉抗生物素蛋白缀合物进行二次探针检测。 HRP 的催化作用导致在探针附近沉积多个生物素基酪酰胺分子，然后可以用标记的链霉抗生物素蛋白缀合物进行检测。检测灵敏度可以是传统检测方法的100倍或更高。

用于酪胺信号放大的试剂

存储类别

11 - Combustible Solids

wgk

WGK 3

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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This study evaluated the sensitivity of biotinyl-tyramide-based in situ hybridization (TISH) method by comparison with chromogenic in situ hybridization (CISH) and immunohistochemical staining (IHC) methods. This study also determined the effect of fixative and fixation time on the detection of

Fluorescence in situ hybridization of scarce leptin receptor mRNA using the enzyme-labeled fluorescent substrate method and tyramide signal amplification.

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To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

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Stress granules (SGs) are cytoplasmic assemblies of proteins and non-translating mRNAs. Whereas much has been learned about SG formation, a major gap remains in understanding the compositional changes SGs undergo during normal disassembly and under disease conditions. Here, we address

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全球贸易项目编号

货号	GTIN
SML2135-250MG	04061835502776
SML2135-50MG	04061835502783