产品名称
ELISA 用 3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物系统, peroxidase substrate
Quality Level
form
liquid
storage temp.
2-8°C
General description
TMB底物是辣根过氧化物酶-HRP的显色底物。
Application
TMB底物可用于利用辣根过氧化物酶结合物进行的ELISA程序。TMB底物会形成可溶的蓝色反应产物，可通过370或655 nm处的吸光度测定。该试剂可与样品中的亚硝酸盐反应，形成紫色的偶氮产物，可在450 nm处测量其吸光度。该反应可利用酸终止，形成黄色反应产物，并可通过450 nm处的光强精确测定。
我们的TMB底物使用方法：
我们的TMB底物使用方法：
- 我们的TMB底物以即用型过氧化物酶底物的形式提供，其中的TMB溶于弱酸性缓冲液中。与过氧化物酶反应之前，底物溶液应呈无色至浅蓝绿色。与过氧化物酶反应时，底物系统形成蓝色反应产物。
- 加入酸终止反应后，测量TMB底物在450 nm处的光强。
- 我们的TMB底物推荐用于ELISA（微孔）程序，且已应用在各种测定方案中，如作为辣根过氧化物酶抗体偶联物的底物，用于检测亨德拉病毒糖蛋白、十四糖、sIgA和嗜中性白细胞胞外阱(NETs)。
- 不建议采用3,3′,5,5′-四甲基联苯胺(TMB)液体底物系统用于膜应用。由于TMB底物会产生可溶性反应产物，因此不建议用于组织化学或印迹分析。
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flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T0440-100ML | 04061838256393 |
| T0440-1L | 04061838256409 |
| T0440-5X1L | 04061838256416 |