T4299
胰蛋白酶-EDTA 溶液
1 ×, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units porcine trypsin and 180 μg EDTA, 4Na per ml in Dulbecco′s PBS without calcium and magnesium
别名:
Cocoonase, Tryptar, Tryptase
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关于此项目
Biological source:
Porcine pancreas
Concentration:
1 ×
biological source
Porcine pancreas
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
impurities
Porcine parvovirus, none detected (9 CFR)
pH
7.0-7.6
shipped in
dry ice
storage temp.
−20°C
Application
该产品的典型用途是使贴壁细胞从培养表面脱落。使细胞从附着面脱落所需的胰蛋白酶浓度主要取决于细胞类型和培养阶段。它可以和内皮细胞培养物一起使用。
Biochem/physiol Actions
胰蛋白酶可切割赖氨酸和精氨酸残基的C端肽。 如果酸性残基位于切割位点的两侧，则该反应的水解速度减慢，而如果脯氨酸残基位于切割位点的羧基侧，则不水解。 胰蛋白酶活性的最佳pH为7-9。 胰蛋白酶还可以切割氨基酸合成衍生物的酯键和酰胺键。 将EDTA作为螯合剂添加到胰蛋白酶溶液中，可中和掩盖胰蛋白酶作用肽键的钙镁离子。 去除这些离子可增加酶活性。
DFP、TLCK、APMSF、AEBSEF和抑肽酶等丝氨酸蛋白酶抑制剂可抑制胰蛋白酶。
DFP、TLCK、APMSF、AEBSEF和抑肽酶等丝氨酸蛋白酶抑制剂可抑制胰蛋白酶。
Other Notes
胰蛋白酶由包含223个氨基酸残基的单链多肽组成，通过去除胰蛋白酶原（在Lys-lle肽键处被切割）中的N-末端六肽而产生。氨基酸序列通过6个二硫键交联。这是胰蛋白酶、β-胰蛋白酶的天然形式。β-胰蛋白酶可以自动裂解，在Lys-Ser残基处切割，产生α-胰蛋白酶。胰蛋白酶是丝氨酸蛋白酶家族的成员。
Disclaimer
本品应置于 -10 至 -40°C 环境冷冻储存。避免反复冻融。
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
实验方案
胰蛋白酶常用于从表面进行贴壁细胞的解离。我们可提供一系列不同的胰蛋白酶溶液来满足您的特定细胞系需求。
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T4299-100ML | 04061837345708 |