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T8787

Triton™ X-100

Molecular Biology

别名:

4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, t-辛基苯氧基聚乙氧基乙醇, 聚乙二醇 叔辛基苯醚

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关于此项目

线性分子式:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
化学文摘社编号:
9036-19-5
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
329826885
MDL number:
MFCD00128254
Beilstein/REAXYS Number:
2315025
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biological source

synthetic (organic)

grade

Molecular Biology

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

aggregation number

100-155

technique(s)

protein purification: suitable, protein quantification: suitable, western blot: suitable

impurities

<1.00% water (Karl Fischer)

pH

9.7

CMC

0.2-0.9 mM (20-25°C)

mp

6 °C

transition temp

cloud point 65 °C, pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

density

1.06 g/mL at 25 °C (lit.)

cation traces

Fe: <5 ppm, K: <0.05%, Na: <0.1%, heavy metals: <5 ppm

HLB

13.5

application(s)

hematology
histology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

General description

Triton™ X-100是一种常用的非离子型的表面活性剂和乳化剂，经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的去污剂，并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。它还可通透活细胞膜用于转染。

Application

Triton™ X-100已用于：
  • 免疫组化用于平面视网膜的染色
  • 与冰冷的PBS（磷酸盐缓冲液）一起悬浮细胞用于细胞DNA分析和Annexin V检测
  • 在免疫荧光显微研究中对细胞进行通透化处理
  • 在LDH检测中作为阳性对照用于测定细胞膜的完整性
  • 采用改良的Belfrage和Vaughan放射性酶法过程测定肝素后血浆中的脂肪酶活性
  • 外层膜蛋白提取物的制备
  • 作为提取缓冲液中一种成分与tris-HCl、NaCl、CaCl2、ZnCl2、Brij 35一起用于小鼠肺细胞的匀浆
  • 免疫荧光标记的组织切片处理

Biochem/physiol Actions

广泛使用的非离子型表面活性剂，用于在温和的非变性条件下回收膜组分。

Features and Benefits

  • 适合灵敏的分子生物学研究
  • 已检测DNA酶和RNA酶
  • 非离子型非变性去污剂
  • 优异的润湿剂和乳化剂
  • 多用表面活性剂

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
如需了解生化试剂系列的更多信息，请填写此表。

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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signalword

Danger

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCJ2105
SLCJ6163
SLCJ0983
SLCH9663
SLCH2982

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Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

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全球贸易项目编号

货号GTIN
T8787-100ML04061838260345
T8787-250ML04061838260352
T8787-50ML04061838260369