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Merck
CN

T9285

Tris-EDTA 缓冲液

100X concentrate for Northern and Southern blotting

别名:

Tris-EDTA洗涤液, TE 缓冲液 溶液

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关于此项目

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
41105319
MDL number:
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grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

pH

7.8-8.2 (1 × in water)

application(s)

sample preparation

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

General description

Tris EDTA缓冲液在分子生物学中常用于很多生物分子沉淀物(包括DNA和RNA)的重悬和保存。其中,Tris-HCl是常用缓冲液,EDTA是金属螯合剂。TE缓冲液可溶解核酸,同时保护其免受降解。可用于核酸分离,比如核酸凝胶电泳、Northern或Southern印迹杂交前的准备。

Application

TE缓冲液已用于:
  • 分离、重悬或保存DNA和RNA用于PCR或qPCR
  • 凝胶电泳,包括Northern和Southern免疫印迹分析
  • 细菌细胞,用于通过AmpC纸片试验检测细胞释放的β-内酰胺酶
  • 免疫组化的抗原修复

Preparation Note

TE缓冲液包含1M Tris-HCl(pH约8.0)和0.1M EDTA。

Other Notes

如需了解生化试剂系列的更多信息,请填写此表


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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Marika Bogdani et al.
Scientific reports, 7(1), 17231-17231 (2017-12-10)
Cystic fibrosis (CF)-related diabetes (CFRD) is thought to result from beta-cell injury due in part to pancreas exocrine damage and lipofibrosis. CFRD pancreata exhibit reduced islet density and altered cellular composition. To investigate a possible etiology, we tested the hypothesis
Detection of vaccinia virus, herpes simplex virus, varicella-zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving: implications for biosafety of bioterrorism agents.
Espy MJ, et al.
Mayo Clinic Proceedings. Mayo Clinic, 77, 624-628 (2002)
Pak Kin Wong et al.
Analytical chemistry, 76(23), 6908-6914 (2004-12-02)
Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor



全球贸易项目编号

货号GTIN
T9285-100ML04061838260468