grade
Molecular Biology
sterility
0.2 μm filtered
form
liquid
pH
7.8-8.2 (1 × in water)
application(s)
sample preparation
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key
VLEIUWBSEKKKFX-UHFFFAOYSA-N
General description
Tris EDTA缓冲液在分子生物学中常用于很多生物分子沉淀物(包括DNA和RNA)的重悬和保存。其中,Tris-HCl是常用缓冲液,EDTA是金属螯合剂。TE缓冲液可溶解核酸,同时保护其免受降解。可用于核酸分离,比如核酸凝胶电泳、Northern或Southern印迹杂交前的准备。
Application
TE缓冲液已用于:
- 分离、重悬或保存DNA和RNA用于PCR或qPCR
- 凝胶电泳,包括Northern和Southern免疫印迹分析
- 细菌细胞,用于通过AmpC纸片试验检测细胞释放的β-内酰胺酶
- 免疫组化的抗原修复
Preparation Note
TE缓冲液包含1M Tris-HCl(pH约8.0)和0.1M EDTA。
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Marika Bogdani et al.
Scientific reports, 7(1), 17231-17231 (2017-12-10)
Cystic fibrosis (CF)-related diabetes (CFRD) is thought to result from beta-cell injury due in part to pancreas exocrine damage and lipofibrosis. CFRD pancreata exhibit reduced islet density and altered cellular composition. To investigate a possible etiology, we tested the hypothesis
Detection of vaccinia virus, herpes simplex virus, varicella-zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving: implications for biosafety of bioterrorism agents.
Espy MJ, et al.
Mayo Clinic Proceedings. Mayo Clinic, 77, 624-628 (2002)
Pak Kin Wong et al.
Analytical chemistry, 76(23), 6908-6914 (2004-12-02)
Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T9285-100ML | 04061838260468 |