W1754
水
PCR Reagent, suitable for PCR
别名:
H2O
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关于此项目
线性分子式:
H2O
化学文摘社编号:
分子量:
18.02
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
产品名称
水, PCR Reagent
vapor density
<1 (vs air)
Quality Level
vapor pressure
3 mmHg
sterility
sterile-filtered
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected, RNase, none detected
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
General description
对 PCR 级水进行无菌过滤。它不含核酸外切酶（dna 酶、rna 酶）和核酸内切酶（切口酶），也不含核酸污染。
Application
水已用于：
- 作为反应混合物的组分和微流控RT-qPCR的稀释剂
- 作为反应混合物的组分，以扩增真菌（杂色云芝）DNA的产物
- 作为稀释剂和反应混合物的组分，以扩增cDNA
- 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积
适用于聚合酶链式反应 (PCR)
Other Notes
便于将水产品规格与 水规格表进行比较。
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存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
实验方案
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
使用逆转录酶和dNTPs进行逆转录（RT）使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。
了解标准 PCR 方案步骤，查看试剂清单或循环参数。本方法使用标准 Taq DNA 聚合酶对 DNA 进行常规 PCR 扩增。
J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| W1754-1VL | 04061837488689 |
| W1754-5VL | 04061837488696 |