Application
由于在缺乏特定培养基成分(比如氨基酸、嘌呤或嘧啶)的情况下无法生长,营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化,可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基,可以提高产量和生长速度,并增加筛选文库或进行基因敲除时转化的成功率。
Preparation Note
1 L 培养基将补充1.92 g。
Other Notes
氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。
补充剂包含以下所有成分,除非另有说明:
氨基酸:除亮氨酸外,所有标准氨基酸的浓度均为76 mg/L,亮氨酸含量为 380 mg/L。
其他营养成分:腺嘌呤(18 mg/L)、肌醇(76 mg/L)、对氨基苯甲酸(8 mg/L)
氨基酸:除亮氨酸外,所有标准氨基酸的浓度均为76 mg/L,亮氨酸含量为 380 mg/L。
其他营养成分:腺嘌呤(18 mg/L)、肌醇(76 mg/L)、对氨基苯甲酸(8 mg/L)
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存储类别
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
实验方案
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
商品
转化是将外源DNA引入细胞,引起遗传变异或基因修饰的过程。1928年,Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
相关内容
Instructions
Xiyan Li et al.
PloS one, 10(12), e0146152-e0146152 (2015-12-31)
Metformin, a leading drug used to treat diabetic patients, is reported to benefit bone homeostasis under hyperglycemia in animal models. However, both the molecular targets and the biological pathways affected by metformin in bone are not well identified or characterized.
Karissa L Cross et al.
mBio, 9(2) (2018-03-15)
The human oral microbiota encompasses representatives of many bacterial lineages that have not yet been cultured. Here we describe the isolation and characterization of previously uncultured Desulfobulbus oralis, the first human-associated representative of its genus. As mammalian-associated microbes rarely have
Dhammika H M L P Navarathna et al.
PloS one, 11(10), e0164449-e0164449 (2016-10-12)
Formation of chlamydospores by Candida albicans was an established medical diagnostic test to confirm candidiasis before the molecular era. However, the functional role and pathological relevance of this in vitro morphological transition to pathogenesis in vivo remain unclear. We compared
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| Y1501-1G | 04061826260470 |
| Y1501-20G | 04061838446732 |