grade
Molecular Biology
Quality Level
usage
kit sufficient for >100 standard transformations
technique(s)
transformation: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Sigma 的酵母转化试剂盒包含了通过醋酸锂方法高效转化酵母的所有必要试剂和对照品。
Application
适用于任何酵母菌株的转化。 方便灵活灵敏，只需 10 ng 的 DNA 即可获得阳性转化子；最佳效率在 0.1-3μg 范围。
Biochem/physiol Actions
用补充突变基因的质粒转化使转化子在缺乏所需成分的培养基上生长。通过在缓冲醋酸锂溶液中孵育，使酵母细胞具有转化能力。然后在含聚乙二醇 (PEG) 的溶液中将细胞与转化 DNA 和载体 DNA 一起孵育进行转化。
Features and Benefits
- 简单易用
- 只需 10 ng 质粒 DNA
- 任何酵母菌株的灵活性
- 足以进行超过 100 次标准转换
Other Notes
酵母转化试剂盒包含：
- 转化缓冲液；100 ml；100 mM 醋酸锂、10 mM Tris HCl (pH 7.6) 和 1 mM EDTA
- 平板缓冲液；100 mL；40%PEG，100 mM 醋酸锂，10 mM Tris HCl，pH 7.5，1 mM EDTA
- 鲑鱼试验的脱氧核糖核酸，10 mg/mL；2 x 1 mL
- 携带 ura 基因的对照酵母质粒 DNA pRS316；10 μg
- 不含尿嘧啶的酵母合成脱落培养基补充剂；1 g
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
法规信息
常规特殊物品
此项目有
实验方案
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
商品
转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Tim R Blower et al.
Nucleic acids research, 47(15), 8163-8179 (2019-07-10)
Type II topoisomerases catalyze essential DNA transactions and are proven drug targets. Drug discrimination by prokaryotic and eukaryotic topoisomerases is vital to therapeutic utility, but is poorly understood. We developed a next-generation sequencing (NGS) approach to identify drug-resistance mutations in
Improved method for high efficiency transformation of intact yeast cells.
D Gietz et al.
Nucleic acids research, 20(6), 1425-1425 (1992-03-25)
Mechanistic and structural insights into the regioselectivity of an acyl-CoA fatty acid desaturase via directed molecular evolution.
Vanhercke T
The Journal of Biological Chemistry, 286(15), 12860-12869 (2011)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| YEAST1-1KT | 04061837588167 |