581325-U
Ascentis® C18 (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
别名:
Ascentis RP18 HPLC色谱柱
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关于此项目
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
100 Å
Matrix:
fully porous particle, silica gel high purity, spherical
产品名称
Ascentis ® C18 HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Level
agency
suitable for USP L1 (Similar to Phenomenex Luna C18)
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
25% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
surface coverage
3.7 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle, silica gel high purity, spherical
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
100 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
General description
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis C18 是特别稳定可靠的首选 HPLC 色谱柱，即使对于难以分离的化合物也能提供对称的峰形和出色的保留性能。
Ascentis C18 是特别稳定可靠的首选 HPLC 色谱柱，即使对于难以分离的化合物也能提供对称的峰形和出色的保留性能。
Application
- Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: 本研究利用 Ascentis® C18 HPLC 色谱柱分离蛋白型胰蛋白酶肽，以鉴定胰岛素类似物，重点介绍了其在靶向质谱法进行蛋白质组学分析中的应用(Qasem RJ et al., 2019).。
- Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: 本研究开发并验证了一种使用 Ascentis® C18 HPLC 色谱柱同时测定多种化合物的 HPLC 方法，证明了其在药物制剂稳定性测试中的稳健性(Byrne J et al., 2014)。
- HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: 本研究采用 Ascentis® C18 HPLC 色谱柱，使用 HPLC-DAD 和 HPLC-ESI-MS/MS 对蜂胶提取物代谢物进行分析，证明了其在复杂天然产物分析中的有效性 (Pellati F et al., 2011)。
- Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: 本文比较了不同的 HPLC 色谱柱技术，包括 Ascentis® C18，重点介绍了其在制药工艺开发中至关重要的快速分离方面的性能 (Abrahim A et al., 2010)。
Features and Benefits
- 出色的保留性能
- 优良的峰形对称性
- 重现性好
- 与 LC-MS 完全兼容
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
-methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon
相关内容
General Care and Use of Supelco™ HPLC Columns
Michael Kühl et al.
eLife, 9 (2020-01-22)
Far-red absorbing chlorophylls are constitutively present as chlorophyll (Chl) d in the cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f, red-shifted phycobiliproteins and minor amounts of Chl d via far-red light photoacclimation in a range of cyanobacteria
Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
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| 货号 | GTIN |
|---|---|
| 581325-U | 04061838548757 |