LOCUS       LV03                    9199 bp    DNA     circular     16-AUG-2017
DEFINITION  .
ACCESSION   urn.local...1z-7x36jcx
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /Recognition_pattern="C^CCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    1..6
                     /Recognition_pattern="CCC^GGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    7..1265
                     /label="EF1a Promoter"
     misc_feature    1266..1271
                     /Recognition_pattern="C^GTACG"
                     /label="BsiWI"
                     /note="Geneious type: restriction site"
     misc_feature    1278..1280
                     /label="Start codon"
     misc_feature    1284..1997
                     /label="GFP"
     misc_feature    2004..2009
                     /Recognition_pattern="A^CGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     misc_feature    2732..2914
                     /label="LTR"
     misc_feature    4080..4940
                     /label="AmpR"
     misc_feature    6394..6576
                     /label="LTR"
     misc_feature    7878..8127
                     /label="U6 Promoter"
     misc_feature    complement(8128..8134)
                     /Recognition_pattern="CACCTGC(4/8)"
                     /label="AarI"
                     /note="Geneious type: restriction site"
     misc_feature    8135..8891
                     /label="LacZ"
     misc_feature    8892..8898
                     /Recognition_pattern="CACCTGC(4/8)"
                     /label="AarI"
                     /note="Geneious type: restriction site"
     misc_feature    8899..8974
                     /label="gRNA scaffold"
ORIGIN      
        1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
      421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
      481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
      541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
      601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
      661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
      721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
      781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
      841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
      901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
      961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     1261 cgtgacgtac ggccaccatg ggcagcgggg gcgaggagct gttcgccggc atcgtgcccg
     1321 tgctgatcga gctggacggc gacgtgcacg gccacaagtt cagcgtgcgc ggcgagggcg
     1381 agggcgacgc cgactacggc aagctggaga tcaagttcat ctgcaccacc ggcaagctgc
     1441 ccgtgccctg gcccaccctg gtgaccaccc tctgctacgg catccagtgc ttcgcccgct
     1501 accccgagca catgaagatg aacgacttct tcaagagcgc catgcccgag ggctacatcc
     1561 aggagcgcac catccagttc caggacgacg gcaagtacaa gacccgcggc gaggtgaagt
     1621 tcgagggcga caccctggtg aaccgcatcg agctgaaggg caaggacttc aaggaggacg
     1681 gcaacatcct gggccacaag ctggagtaca gcttcaacag ccacaacgtg tacatccgcc
     1741 ccgacaaggc caacaacggc ctggaggcta acttcaagac ccgccacaac atcgagggcg
     1801 gcggcgtgca gctggccgac cactaccaga ccaacgtgcc cctgggcgac ggccccgtgc
     1861 tgatccccat caaccactac ctgagcactc agaccaagat cagcaaggac cgcaacgagg
     1921 cccgcgacca catggtgctc ctggagtcct tcagcgcctg ctgccacacc cacggcatgg
     1981 acgagctgta cagggcttga tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     2041 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2101 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2161 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2221 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2281 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     2341 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2401 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     2461 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     2521 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     2581 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     2641 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     2701 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     2761 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2821 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2881 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     2941 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3061 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3121 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3181 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3241 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     3301 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     3361 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3421 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3481 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3541 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3781 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3841 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     3961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4261 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4321 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4381 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4621 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4681 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4921 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5221 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5761 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5821 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5881 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     5941 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6001 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6061 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     6121 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     6181 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     6241 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     6301 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     6361 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     6421 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6481 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6541 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     6601 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     6661 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     6721 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     6781 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     6841 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     6901 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     6961 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     7021 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     7081 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     7141 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     7201 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     7261 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     7321 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7381 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     7441 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7501 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     7561 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     7621 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     7681 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     7741 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     7801 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     7861 accccgaggg gacccagaga gggcctattt cccatgattc cttcatattt gcatatacga
     7921 tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta
     7981 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     8041 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     8101 tatatcttgt ggaaaggacg aaacaccgca ggtgaatacg caaaccgcct ctccccgcgc
     8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8341 gctatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8401 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8461 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8521 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
     8581 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
     8641 atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat
     8701 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag
     8761 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg
     8821 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc
     8881 gccggagaaa acacctgcgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     8941 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt aagcttggcg taactagatc
     9001 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     9061 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     9121 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     9181 tggcgccggc tcgaggggg
//
