LOCUS       LV04                    8671 bp    DNA     circular     19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...24-7x36jda
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    468..503
                     /vntifkey="21"
                     /label=Psi
     LTR             complement(555..735)
                     /vntifkey="19"
                     /label=LTR_1
     rep_origin      1513..1982
                     /vntifkey="33"
                     /label=F1ori
     misc_feature    2099..2959
                     /vntifkey="21"
                     /label=Amp
     rep_origin      3115..3777
                     /vntifkey="33"
                     /label=pUC\ori
     polyA_signal    4500..4724
                     /vntifkey="25"
                     /label=PolyA\signal
     LTR             complement(4750..5005)
                     /vntifkey="19"
                     /label=LTR_2
     CDS             complement(5085..5786)
                     /vntifkey="4"
                     /label=tagBFP
     misc_feature    5787..5840
                     /vntifkey="21"
                     /label=2A
     CDS             complement(5859..6455)
                     /vntifkey="4"
                     /label=Puro
     misc_feature    complement(6495..6994)
                     /vntifkey="21"
                     /label=hPGK
                     /note="Geneious type: promoter uukaryotic"
     misc_feature    7027..7267
                     /vntifkey="21"
                     /label=U6\Promoter
                     /note="Geneious type: promoter uukaryotic"
     misc_feature    complement(7266..7271)
                     /Recognition_pattern="GAAGAC(2/6)"
                     /vntifkey="21"
                     /label=BbsI
                     /note="Geneious type: restriction site"
     misc_feature    7280..7382
                     /vntifkey="21"
                     /label=gRNA
     misc_feature    7296..7301
                     /Recognition_pattern="GAAGAC(2/6)"
                     /vntifkey="21"
                     /label=BbsI
                     /note="Geneious type: restriction site"
     misc_feature    8487..8546
                     /vntifkey="21"
                     /label=RRE
     misc_feature    4179..4213
                     /vntifkey="21"
                     /label=PB-Left
     misc_feature    7553..7615
                     /vntifkey="21"
                     /label=PB-Right
     misc_feature    7738..7797
                     /vntifkey="21"
                     /label=cPPT
BASE COUNT     2009 a      2235 c      2119 g      2308 t 
ORIGIN
        1 tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg 
       61 tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct 
      121 ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt 
      181 ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg 
      241 tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat 
      301 gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc 
      361 taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc 
      421 tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt 
      481 cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc 
      541 tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga gggatctcta 
      601 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta 
      661 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc 
      721 taaccagaga gacccagtac aggcaaaacg cgctgcttat atagacctcc caccgtacac 
      781 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt 
      841 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc 
      901 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa 
      961 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact 
     1021 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca 
     1081 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac 
     1141 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg 
     1201 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga 
     1261 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca 
     1321 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg 
     1381 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 
     1441 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 
     1501 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
     1561 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
     1621 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 
     1681 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 
     1741 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
     1801 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 
     1861 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 
     1921 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 
     1981 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
     2041 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 
     2101 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 
     2161 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 
     2221 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 
     2281 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 
     2341 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 
     2401 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 
     2461 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 
     2521 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 
     2581 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 
     2641 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 
     2701 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 
     2761 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 
     2821 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 
     2881 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 
     2941 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 
     3001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 
     3061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 
     3121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 
     3181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 
     3241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 
     3301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 
     3361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 
     3421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 
     3481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 
     3541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 
     3601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 
     3661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 
     3721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 
     3781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
     3841 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
     3901 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
     3961 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 
     4021 cactcattag gcaccccagg ctttacactt tatgctcccg gctcgtatgt tgtgtggaat 
     4081 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc 
     4141 aattaaccct cactaaaggg aacaaaagct taatttaacc ctagaaagat agtctgcgta 
     4201 aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg 
     4261 ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg 
     4321 cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta 
     4381 tcttttacgt gacttttaag atttaactca tacgataatt atattgttat ttcatgttct 
     4441 acttacgtga taacttatta tatatatatt ttcttgttat agatatcatt aacggccgcc 
     4501 catagagccc accgcatccc cagcatgcct gctattgact tcccaatcct cccccttgct 
     4561 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat 
     4621 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac 
     4681 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg 
     4741 ggtttaaact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 
     4801 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 
     4861 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 
     4921 agagagactc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgacttct 
     4981 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac 
     5041 agctgccttg taagtcattg gtcttaaagg tacctagcgg ccgctcaatt aagcttgtgc 
     5101 cccagtttgc tagggaggtc gcagtatctg gccactgcca cctcgtgctg ctcgacgtag 
     5161 gtctcgttgt tggcctcctt gattctttcc agtctgtagt ccacatagta gacgccaggc 
     5221 atcttgaggt tcttagcggg tttcttggat ctatatgtgg tcttgatgtt tgcgatcaga 
     5281 tggctcccgc ccacgagctt cagggccatg tcgtttctgc cttccaggcc gccgtcagcg 
     5341 gggtacagcg tctcggtgaa ggcctcccag ccgagtgttt tcttctgcat cacagggccg 
     5401 ttggatgtga agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg 
     5461 ctggtgtcct gggtagcggt cagcacgccc ccgtcctcgt atgtggtgac tctctcccat 
     5521 gtgaagccct cagggaagga ctgcttgaag aagtcgggga tgccctgggt gtggttgatg 
     5581 aaggtcttgc tgccgtagag gaagctagta gccaggatgt cgaaggcgaa ggggagaggg 
     5641 ccgccctcga ccaccttgat tctcatggtc tgggtgccct cgtagggctt gccttcgccc 
     5701 tcggatgtgc acttgaagtg atggttgtcc acggtgccct ccatgtacag cttcatgtgc 
     5761 atgttctcct taatcagctc gctcattggg ccaggattct cctccacgtc accgcatgtt 
     5821 aggagacttc ctctgccctc tcctccggac ccgccgccgg caccgggctt gcgggtcatg 
     5881 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc 
     5941 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc 
     6001 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg 
     6061 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg 
     6121 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg 
     6181 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc 
     6241 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag 
     6301 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg 
     6361 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg 
     6421 gtggcgaggc gcaccgtggg cttgtactcg gtcatggtaa gcttcagctg ctcgagatct 
     6481 agatggatgc aggtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg 
     6541 cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc 
     6601 ctgagcccgc ccctgagccc gcccccggac ccaccccttc ccagcctctg agcccagaaa 
     6661 gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc ctacccgctt ccattgctca 
     6721 gcggtgctgt ccatctgcac gagactagtg agacgtgcta cttccatttg tcacgtcctg 
     6781 cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc 
     6841 gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt ggaatgtgtg 
     6901 cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct 
     6961 ccagactgcc ttgggaaaag cgcctcccct acccggtaga attggatcgc caaggtcggg 
     7021 caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt 
     7081 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga 
     7141 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac 
     7201 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg 
     7261 aaacagtctt catgcggatc cattagaatt catatgaaga ctttagagct agaaatagca 
     7321 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt 
     7381 ttgctttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa 
     7441 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact 
     7501 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc 
     7561 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaag 
     7621 taacgcgtag ggcccggtac taaccaaact ggatctctgc tgtccctgta ataaacccga 
     7681 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt 
     7741 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg 
     7801 tggatgaata ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa 
     7861 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc 
     7921 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac 
     7981 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat 
     8041 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact 
     8101 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc 
     8161 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca 
     8221 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac 
     8281 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt 
     8341 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag 
     8401 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat 
     8461 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct 
     8521 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct 
     8581 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac 
     8641 tttatattta tataattcac ttctccaatt g 
//
