LOCUS       LV05                   13757 bp    DNA     circular     26-SEP-2017
DEFINITION  .
ACCESSION   urn.local...4f-83tth5k
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CRISPR_gRNA     606..1017
                     /created_by="cjowdy"
                     /X-Transferred_From="LV01; LV02"
                     /X-Transferred_Similarity="100.00%; 60.44%"
                     /modified_by="cjowdy"
                     /Transferred_From="LV01, LV02, LV06, LV04"
                     /Transferred_Similarity="56.55% -> 54.85%"
                     /label="RSV 5' LTR"
     misc_binding    1621..1972
                     /Color="#ff55ff"
                     /modified_by="cjowdy"
                     /X-Transferred_From="LV01; LV02; LV03; LV04"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV01, LV02, LV03, LV04, LV06"
                     /Transferred_Similarity="100.00%"
                     /label="RRE"
     misc_feature    2440..2557
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /X-Transferred_From="LV01; LV02; LV03; LV06; LV07; pLKO.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV01, LV02, LV03, LV04, LV06, LV07"
                     /Transferred_Similarity="100.00%"
                     /Color="#1199ff"
                     /modified_by="cjowdy"
                     /label="cPPT"
     promoter        2608..2848
                     /note="RNA polymerase III promoter for human U6 snRNA; U6
                     promoter"
                     /X-Transferred_From="Exported; LV02; LV03"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV02, LV03, LV06"
                     /Transferred_Similarity="100.00%"
                     /label="U6 promoter"
     misc_feature    3608..3710
                     /Transferred_From="LV04"
                     /Transferred_Similarity="82.52%"
                     /label="gRNA"
     promoter        3734..3984
                     /Color="#19a0ff"
                     /modified_by="cjowdy"
                     /X-Transferred_From="LV01"
                     /X-Transferred_Similarity="93.63%"
                     /Transferred_From="LV06, LV01"
                     /Transferred_Similarity="96.41% -> 94.02%"
                     /label="EF1a"
     mat_peptide     3995..8119
                     /modified_by="cjowdy"
                     /Transferred_From="LV01"
                     /Transferred_Similarity="96.78%"
                     /label="Cas9"
     misc_feature    8147..8170
                     /label="Flag tag"
     misc_feature    8171..8236
                     /label="P2A"
     unsure          8237..8830
                     /modified_by="cjowdy"
                     /X-Transferred_From="LV01; LV04"
                     /X-Transferred_Similarity="99.66% -> 99.49%; 99.83%"
                     /Transferred_From="LV04, LV06, LV01"
                     /Transferred_Similarity="99.83% -> 99.66%"
                     /label="PuroR"
     misc_feature    8849..9437
                     /note="WPRE; woodchuck hepatitis virus posttranscriptional
                      regulatory element"
                     /X-Transferred_From="Exported; LV02"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV02, LV03"
                     /Transferred_Similarity="100.00%"
                     /label="WPRE"
     LTR             9509..9742
                     /note="3' LTR (Delta-U3); LTR (Delta-U3);
                     self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1; self-inactivating long terminal repeat (LTR) from 
                     HIV-1"
                     /X-Transferred_From="Exported; LV02; LV03;
                     Lv5-tEF1a-Cas9-G; pLKO.1"
                     /X-Transferred_Similarity="100.00%"
                     /Color="#888888"
                     /modified_by="cjowdy"
                     /Transferred_From="LV01, LV02, LV03"
                     /Transferred_Similarity="100.00%"
                     /label="SIN 3' LTR; SIN 3' LTR "
     misc_feature    10044..10513
                     /X-Transferred_From="LV01; LV02; LV04"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV01, LV02, LV04, LV06"
                     /Transferred_Similarity="93.83%"
                     /label="f1 ori"
     rep_origin      complement(12005..12593)
                     /note="RNaseH cleavage point; high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication; ori; pMB1
                     ori; pMB1 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point); pMB1 origin of replication
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point);
                     pUC19 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point); pUC19 origin of replication
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point)"
                     /direction="LEFT; RIGHT"
                     /Color="#888888"
                     /modified_by="cjowdy"
                     /X-Transferred_From="Exported; LITMUS 28i; LITMUS 38i;
                     LV01; LV02; LV03; LV04; Lv5-tEF1a-Cas9-G; pGPS3; pLKO.1;
                     pNEB193; pNEB205A; pNEB206A; pNEBR-X1; pUC19"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV01, LV02, LV03, LV04, LV06"
                     /Transferred_Similarity="100.00%"
                     /label="pUC ori"
     CDS             complement(12764..13624)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR; ampR (confers resistance to ampicillin);
                     confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /Color="#888888"
                     /modified_by="cjowdy"
                     /X-Transferred_From="Exported; LV01; LV02;
                     Lv5-tEF1a-Cas9-G; pCRII-TOPO; pFOS1; pGPS3; pKLAC1;
                     pLKO.1; pNEB193; pNEB205A; pNEB206A; pNEBR-R1; pRS413;
                     pUC19"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW"
                     /Transferred_From="LV01, LV02, LV06"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR"
ORIGIN      
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccgcag gtgaatacgc aaaccgcctc
     2881 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2941 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     3001 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     3061 caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac
     3121 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3181 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3241 ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag
     3301 tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac
     3361 gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc
     3421 acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag
     3481 gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac
     3541 gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca
     3601 tttttacgcg ccggagaaaa cacctgcgtt ttagagctag aaatagcaag ttaaaataag
     3661 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ctagctaggt
     3721 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     3781 agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg
     3841 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     3901 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     3961 cagaacacag gaccggttct agagcgctgc caccatggac aagaagtaca gcatcggcct
     4021 ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
     4081 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     4141 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
     4201 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     4261 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     4321 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     4381 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     4441 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     4501 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
     4561 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
     4621 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
     4681 gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac
     4741 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
     4801 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
     4861 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
     4921 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
     4981 ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga
     5041 gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca
     5101 ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact
     5161 gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag
     5221 catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt
     5281 ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc
     5341 ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag
     5401 cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca
     5461 gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc
     5521 caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata
     5581 cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt
     5641 ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt
     5701 caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc
     5761 ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa
     5821 tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag
     5881 agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa
     5941 gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg
     6001 catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc
     6061 caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca
     6121 gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg
     6181 cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa
     6241 agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac
     6301 cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa
     6361 agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga
     6421 gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga
     6481 catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga
     6541 cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa
     6601 cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc
     6661 caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag
     6721 cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa
     6781 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct
     6841 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     6901 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     6961 gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     7021 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     7081 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     7141 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     7201 cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat
     7261 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     7321 tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa
     7381 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     7441 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     7501 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     7561 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     7621 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     7681 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     7741 cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat
     7801 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     7861 agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat
     7921 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     7981 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     8041 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacaa
     8101 gcgacctgcc gccacaaaga aggctggaca ggctaagaag aagaaagatt acaaagacga
     8161 tgacgataag ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     8221 agagaatcct ggaccgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     8281 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     8341 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8401 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     8461 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8521 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8581 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     8641 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     8701 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     8761 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     8821 gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     8881 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8941 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     9001 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     9061 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     9121 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     9181 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     9241 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     9301 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     9361 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     9421 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     9481 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     9541 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     9601 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9661 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9721 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     9781 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     9841 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     9901 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     9961 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
    10021 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    10081 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    10141 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    10201 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    10261 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    10321 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    10381 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    10441 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
    10501 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    10561 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10621 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    10681 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10741 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10801 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
    10861 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
    10921 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
    10981 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
    11041 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
    11101 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
    11161 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
    11221 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
    11281 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
    11341 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    11401 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    11461 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    11521 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    11581 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    11641 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    11701 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    11761 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    11821 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    11881 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    11941 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    12001 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    12061 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    12121 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    12181 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    12241 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    12301 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    12361 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    12421 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    12481 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    12541 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    12601 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    12661 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    12721 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    12781 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    12841 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    12901 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    12961 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    13021 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    13081 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    13141 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    13201 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    13261 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    13321 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    13381 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    13441 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    13501 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    13561 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    13621 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    13681 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    13741 gaaaagtgcc acctgac
//
