LOCUS       LV07                    8161 bp    DNA     circular     26-SEP-2017
DEFINITION  .
ACCESSION   urn.local...q-7xzi2zr
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..1265
                     /Color="#19a0ff"
                     /modified_by="cjowdy"
                     /X-Transferred_From="LV02"
                     /X-Transferred_Similarity="99.92%"
                     /Transferred_From="LV06"
                     /Transferred_Similarity="100.00%"
                     /label="EF1a Promoter"
     CDS             1272..1646
                     /label="Zeo"
     misc_feature    1671..2259
                     /note="WPRE; woodchuck hepatitis virus posttranscriptional
                      regulatory element"
                     /X-Transferred_From="LV02; LV03"
                     /X-Transferred_Similarity="99.83%"
                     /Transferred_From="LV06"
                     /Transferred_Similarity="100.00%"
                     /label="WPRE"
     LTR             2331..2564
                     /note="3' LTR (Delta-U3); LTR (Delta-U3);
                     self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1; self-inactivating long terminal repeat (LTR) from 
                     HIV-1"
                     /X-Transferred_From="LV01; LV02; LV03; LV04"
                     /X-Transferred_Similarity="99.57% -> 87.61%"
                     /Color="#888888"
                     /modified_by="cjowdy"
                     /Transferred_From="LV06"
                     /Transferred_Similarity="100.00%"
                     /label="SIN 3' LTR"
     CDS             3731..4591
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR; ampR (confers resistance to ampicillin);
                     confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /Color="#888888"
                     /modified_by="cjowdy"
                     /X-Transferred_From="Exported; LV01; LV02; LV06;
                     Lv5-tEF1a-Cas9-G; pCRII-TOPO; pFOS1; pGPS3; pKLAC1;
                     pLKO.1; pNEB193; pNEB205A; pNEB206A; pNEBR-R1; pRS413;
                     pUC19"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW"
                     /Transferred_From="LV01, LV02, LV05, LV06"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR"
     rep_origin      4762..5350
                     /note="RNaseH cleavage point; high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication; ori; pMB1
                     ori; pMB1 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point); pMB1 origin of replication
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point);
                     pUC19 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point); pUC19 origin of replication
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point)"
                     /direction="LEFT; RIGHT"
                     /Color="#888888"
                     /modified_by="cjowdy"
                     /X-Transferred_From="Exported; LITMUS 28i; LITMUS 38i;
                     LV01; LV02; LV03; LV04; LV06; Lv5-tEF1a-Cas9-G; pGPS3;
                     pLKO.1; pNEB193; pNEB205A; pNEB206A; pNEBR-X1; pUC19"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV01, LV02, LV03, LV04, LV05, LV06"
                     /Transferred_Similarity="100.00%"
                     /label="pUC ori"
     misc_structure  5817..6047
                     /X-Transferred_From="LV01"
                     /X-Transferred_Similarity="100.00%"
                     /Color="#777777"
                     /modified_by="cjowdy"
                     /Transferred_From="LV03"
                     /Transferred_Similarity="100.00%"
                     /label="RSV 5' LTR"
     misc_binding    6832..7183
                     /Color="#ff55ff"
                     /modified_by="cjowdy"
                     /X-Transferred_From="LV01; LV02; LV03; LV04; LV06"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV01, LV02, LV03, LV04, LV05, LV06"
                     /Transferred_Similarity="100.00%"
                     /label="RRE"
     promoter        7531..7771
                     /note="RNA polymerase III promoter for human U6 snRNA; U6
                     promoter"
                     /X-Transferred_From="Exported; LV02; LV03; LV06"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LV02, LV03, LV05, LV06"
                     /Transferred_Similarity="100.00%"
                     /label="U6 promoter"
     misc_feature    7804..7942
                     /label="gRNA scaffold"
     misc_feature    7992..8109
                     /Color="#1199ff"
                     /modified_by="cjowdy"
                     /label="cPPT"
ORIGIN      
        1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
      421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
      481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
      541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
      601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
      661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
      721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
      781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
      841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
      901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
      961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     1261 cgtgacgtac aatggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     1321 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     1381 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     1441 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     1501 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     1561 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     1621 acttcgtggc cgaggagcag gactgagaat tcgatatcaa gcttatcggt aatcaacctc
     1681 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     1741 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     1801 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     1861 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     1921 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     1981 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2041 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2101 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2161 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgactt cgccttcgcc
     2221 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     2281 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     2341 aattcactcc caacgaagtc aagatctgct ttttgcttgt actgggtctc tctggttaga
     2401 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2461 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2521 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     2581 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     2641 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2761 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     2821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2881 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2941 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     3001 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3061 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3121 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3181 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3241 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3301 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3361 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3421 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3481 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3541 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3901 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3961 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4021 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4081 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4141 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4201 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4261 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4321 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4381 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4441 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4501 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4561 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4621 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4681 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5221 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5281 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5341 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5401 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5461 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5521 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     5581 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5641 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5701 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     5761 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     5821 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     5881 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     5941 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     6001 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     6061 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6121 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6181 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     6241 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     6301 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     6361 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     6421 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     6481 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     6541 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     6601 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     6661 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     6721 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     6781 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     6841 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     6901 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     6961 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     7021 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     7081 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     7141 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     7201 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     7261 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     7321 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     7381 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     7441 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     7501 acccacctcc caaccccgag gggacccaga gagggcctat ttcccatgat tccttcatat
     7561 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca
     7621 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
     7681 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
     7741 ttcttggctt tatatatctt gtggaaagga cgaaacaccg nnnnnnnnnn nnnnnnnnng
     7801 ttttagagct aggccaacat gaggatcacc catgtctgca gggcctagca agttaaaata
     7861 aggctagtcc gttatcaact tggccaacat gaggatcacc catgtctgca gggccaagtg
     7921 gcaccgagtc ggtgcttttt ttaagcttgg cgtaactaga tcttgagaca aatggcagta
     7981 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     8041 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     8101 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg
     8161 g
//
