LOCUS       p02                     8270 bp    DNA     circular     22-AUG-2017
DEFINITION  .
ACCESSION   urn.local...2o-7x36jev
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    35..430
                     /label="U6gRNA"
     misc_feature    437..448
                     /label="Vector Identifier"
     misc_feature    457..1036
                     /label="CMV"
     misc_feature    1132..5253
                     /label="Cas9"
     misc_feature    5260..5328
                     /label="2A"
     misc_feature    5329..6051
                     /label="RFP"
     misc_feature    6076..6083
                     /Recognition_pattern="GTTT^AAAC"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI, MssI"
                     /note="Geneious type: restriction site"
     polyA_signal    6101..6325
                     /label="BGH pA"
     misc_feature    6498..7292
                     /label="KanR"
     rep_origin      7591..8264
                     /label="pUC ori"
ORIGIN      
        1 gctgcttcgc gatgtacggg ccagatatac gcgtaaggtc gggcaggaag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc
      301 aggtggacac ctgcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      361 caacttgaaa aagtggcacc gagtcggtgc ttttttctag gcccagcttt cttgtacaaa
      421 gttggcatta gtcgacatcc aagcaacctt aattaatgac attgattatt gactagttat
      481 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      541 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      601 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      661 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      721 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      781 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      841 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      901 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      961 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     1021 gaggtctata taagcaacca ggttctggct aactagagaa cccactgctt actggcttat
     1081 cgaaattaat acgactcact atagggagac ccaagctggc tagccgccac catggacaag
     1141 aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac
     1201 gactacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
     1261 aagaagaacc tgatcggcgc cctgctgttc ggctctggcg aaacagccga ggccacccgg
     1321 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
     1381 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
     1441 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
     1501 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaagctggcc
     1561 gacagcaccg acaaggccga cctgagactg atctacctgg ccctggccca catgatcaag
     1621 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     1681 ctgttcatcc agctggtgca gatctacaat cagctgttcg aggaaaaccc catcaacgcc
     1741 agcagagtgg acgccaaggc catcctgagc gccagactga gcaagagcag acggctggaa
     1801 aatctgatcg cccagctgcc cggcgagaag cggaatggcc tgttcggcaa cctgattgcc
     1861 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa
     1921 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc
     1981 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc
     2041 gacatcctga gagtgaacag cgagatcacc aaggcccccc tgtccgcctc tatgatcaag
     2101 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg
     2161 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatc
     2221 gatggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg
     2281 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg
     2341 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg
     2401 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc
     2461 ctgaccttca gaatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc
     2521 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac
     2581 aagggcgcca gcgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc
     2641 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtacaacgag
     2701 ctgaccaaag tgaaatacgt gaccgaggga atgcggaagc ccgcctttct gagcggcgag
     2761 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag
     2821 ctgaaagagg actacttcaa gaaaatcgag tgcttcgaca gcgtggaaat cagcggcgtg
     2881 gaagatcggt tcaacgcctc cctgggcgcc tatcacgatc tgctgaaaat tatcaaggac
     2941 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
     3001 acactgtttg aggaccgggg catgatcgag gaacggctga aaacctatgc ccacctgttc
     3061 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc
     3121 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
     3181 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
     3241 tttaaagagg acatccagaa agcccaggtg tccggccagg gacactctct gcacgagcag
     3301 atcgccaatc tggccggatc ccccgccatt aagaagggca tcctgcagac agtgaagatt
     3361 gtggacgagc tcgtgaaagt gatgggccac aagcccgaga acatcgtgat cgaaatggcc
     3421 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     3481 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     3541 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     3601 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccacat tgtgccccag
     3661 tccttcatca aggacgactc catcgataac aaagtgctga ctcggagcga caagaaccgg
     3721 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgc
     3781 cagctgctga atgccaagct gattacccag aggaagttcg acaatctgac caaggccgag
     3841 agaggcggcc tgagcgaact ggataaggcc ggcttcatta agcggcagct ggtggaaacc
     3901 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     3961 gagaacgaca aactgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     4021 gacttcagaa aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     4081 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     4141 gaaagcgagt tcgtgtacgg cgattacaag gtgtacgacg tgcggaagat gatcgccaag
     4201 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     4261 tttttcaaga ccgagatcac actggccaac ggcgagatca gaaagcggcc tctgatcgag
     4321 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc cacagtgcgg
     4381 aaagtgctgt ccatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gaccggcggc
     4441 ttcagcaaag agtctatcct gcccaagagg aactccgaca agctgatcgc cagaaagaag
     4501 gattgggacc ctaagaagta cggcggcttt gacagcccca ccgtggccta ctctgtgctg
     4561 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     4621 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     4681 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     4741 gagctggaaa acggccggaa gcggatgctg gcttctgccg gcgaactgca gaagggaaac
     4801 gagctggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     4861 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     4921 tacctggacg agatcatcga gcagattagc gagttctcca agcgcgtgat cctggccgat
     4981 gccaacctgg acaaggtgct gagcgcctac aacaagcacc gggataagcc catcagagag
     5041 caggccgaga atatcatcca cctgtttacc ctgaccaacc tgggagcccc tgccgccttc
     5101 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
     5161 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     5221 ctgggaggcg accccaagaa aaagcgcaaa gtggttaacg gcagtggaga gggcagagga
     5281 agtctgctaa catgcggtga cgtcgaggag aatcctggcc cacgatcgat gcatgtgtct
     5341 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     5401 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     5461 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     5521 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     5581 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     5641 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     5701 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     5761 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     5821 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     5881 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     5941 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     6001 agatactgcg acctccctag caaactgggg cacaaactta atgctgttaa ctgatgactc
     6061 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6121 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6181 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6241 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6301 gctggggatg cggtgggctc tatggcttct actgggcggt tttatggaca gcaagcgaac
     6361 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
     6421 tggctttctt gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag
     6481 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6541 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6601 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6661 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6721 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6781 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6841 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6901 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6961 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7021 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7081 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7141 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7201 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7261 ccttctatcg ccttcttgac gagttcttct gaattattaa cgcttacaat ttcctgatgc
     7321 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg
     7381 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7441 ctcatgagac aataaccctg ataaatgctt caataatagc acgtgctaaa acttcatttt
     7501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7741 agagcgcaga taccaaatac tgtctttcta gtgtagccgt agttaggcca ccacttcaag
     7801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
//
