LOCUS       p03                     2400 bp    DNA     circular     22-AUG-2017
DEFINITION  .
ACCESSION   urn.local...2t-7x36jf6
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    173..967
                     /label="Kan R"
     misc_feature    1266..1939
                     /label="pUC Ori"
     misc_feature    1980..2243
                     /label="U6"
     misc_feature    complement(2241..2246)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    2309..2314
                     /Recognition_pattern="CGTCTC(1/5)"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    2312..2394
                     /label="sgRNA scaffold"
ORIGIN      
        1 cttctactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
       61 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
      121 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga
      181 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
      241 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
      301 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
      361 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
      421 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
      481 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
      541 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
      601 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
      661 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
      721 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
      781 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
      841 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
      901 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
      961 cttctgaatt attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     1021 cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1081 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1141 tgcttcaata atagcacgtg ctaaaacttc atttttaatt taaaaggatc taggtgaaga
     1201 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1921 gctggccttt tgctcacatg ttcttgctgc ttcgcgatgt acgggccaga tatgaattca
     1981 aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca
     2041 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
     2101 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     2161 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     2221 tcttgtggaa aggacgaaat gagacgtcag ccatgattaa gcaagcgctc aagcttcacc
     2281 ggtttcgaag caacggaatt catgactgcg tctcttagag ctagaaatag caagttaaaa
     2341 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttaagctt
//
