LOCUS       p04                     8348 bp    DNA     circular     22-AUG-2017
DEFINITION  .
ACCESSION   urn.local...2e-7x36jdy
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    35..430
                     /label="U6gRNA"
     misc_feature    82..101
                     /label="gRNA SeqPrimer"
     misc_feature    432..459
                     /label="Vector Tag"
     misc_feature    463..1042
                     /label="CMV"
     misc_feature    1093..1112
                     /label="T7"
     misc_feature    1141..1206
                     /label="3xFlag"
     misc_feature    1207..5307
                     /label="eCas91.1"
     misc_feature    5308..5328
                     /label="NLS"
     misc_feature    5335..5403
                     /label="2A"
     misc_feature    5410..6123
                     /label="GFP"
     polyA_signal    6179..6403
                     /label="BGH pA"
     misc_feature    6361..6366
                     /Recognition_pattern="GAAGAC(2/6)"
                     /label="BbsI"
                     /note="Geneious type: restriction site"
     misc_feature    6576..7370
                     /label="KanR"
     rep_origin      7669..8342
                     /label="pUC ori"
ORIGIN      
        1 gctgcttcgc gatgtacggg ccagatatac gcgtaaggtc gggcaggaag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc
      301 aggtggacac ctgcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      361 caacttgaaa aagtggcacc gagtcggtgc ttttttctag gcccagcttt cttgtacaaa
      421 gttggcatta gtcgacaagc acagctgagc aaccttaatt aatgacattg attattgact
      481 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      541 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      601 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      661 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      721 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      781 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      841 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      901 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      961 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1021 cggtgggagg tctatataag caaccaggtt ctggctaact agagaaccca ctgcttactg
     1081 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc cgccaccatg
     1141 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac
     1201 gataaggaca agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc
     1261 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
     1321 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
     1381 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
     1441 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
     1501 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
     1561 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
     1621 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
     1681 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
     1741 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
     1801 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
     1861 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga
     1921 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
     1981 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
     2041 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
     2101 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
     2161 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
     2221 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
     2281 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc
     2341 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg
     2401 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
     2461 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag
     2521 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac
     2581 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag
     2641 gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat
     2701 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc
     2761 gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc
     2821 ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg
     2881 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa
     2941 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa
     3001 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc
     3061 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat
     3121 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg
     3181 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc
     3241 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac
     3301 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc
     3361 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag
     3421 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc
     3481 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag
     3541 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac
     3601 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg
     3661 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac
     3721 catatcgtgc ctcagagctt tctggcggac gactccatcg acaacaaggt gctgaccaga
     3781 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg
     3841 aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat
     3901 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga
     3961 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg
     4021 aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag
     4081 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac
     4141 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa
     4201 aagtaccctg cgctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg
     4261 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac
     4321 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag
     4381 gcgcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat
     4441 tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag
     4501 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg
     4561 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg
     4621 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt
     4681 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc
     4741 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct
     4801 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa
     4861 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc
     4921 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg
     4981 gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga
     5041 gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat
     5101 aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga
     5161 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc
     5221 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg
     5281 atcgacctgt ctcagctggg aggcgacccc aagaaaaagc gcaaagtggt taacggcagt
     5341 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccacga
     5401 tcgatgcata gcgggggcga ggagctgttc gccggcatcg tgcccgtgct gatcgagctg
     5461 gacggcgacg tgcacggcca caagttcagc gtgcgcggcg agggcgaggg cgacgccgac
     5521 tacggcaagc tggagatcaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5581 accctggtga ccaccctctg ctacggcatc cagtgcttcg cccgctaccc cgagcacatg
     5641 aagatgaacg acttcttcaa gagcgccatg cccgagggct acatccagga gcgcaccatc
     5701 cagttccagg acgacggcaa gtacaagacc cgcggcgagg tgaagttcga gggcgacacc
     5761 ctggtgaacc gcatcgagct gaagggcaag gacttcaagg aggacggcaa catcctgggc
     5821 cacaagctgg agtacagctt caacagccac aacgtgtaca tccgccccga caaggccaac
     5881 aacggcctgg aggctaactt caagacccgc cacaacatcg agggcggcgg cgtgcagctg
     5941 gccgaccact accagaccaa cgtgcccctg ggcgacggcc ccgtgctgat ccccatcaac
     6001 cactacctga gcactcagac caagatcagc aaggaccgca acgaggcccg cgaccacatg
     6061 gtgctcctgg agtccttcag cgcctgctgc cacacccacg gcatggacga gctgtacagg
     6121 gctgttaact gatgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     6181 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6241 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6301 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6361 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctac tgggcggttt
     6421 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
     6481 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
     6541 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     6601 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6661 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6721 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     6781 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6841 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6901 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6961 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7021 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7081 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     7141 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7201 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7261 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7321 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg
     7381 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     7441 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7501 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatagcac
     7561 gtgctaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7801 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8341 atgttctt
//
