LOCUS       pVAX-Cas9\Nuclea        8343 bp    DNA     circular SYN  4-APR-2013
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Mark Gerber
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2018 from SnapGene Viewer 4.0.5 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     http://www.informaxinc.com/
            ORIGDB|GenBank
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
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COMMENT     VNTDBDATE|658368000|
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FEATURES             Location/Qualifiers
     misc_feature    82..101
                     /vntifkey="21"
                     /label=gRNA\SeqPrimer
                     /note="/vntifkey=21"
     misc_feature    441..452
                     /vntifkey="21"
                     /label=Vector\Identifier
                     /note="/vntifkey=21"
     misc_feature    461..1040
                     /vntifkey="21"
                     /label=CMV
                     /note="/vntifkey=21"
     misc_feature    1091..1110
                     /vntifkey="21"
                     /label=T7
                     /note="/vntifkey=21"
     misc_feature    1139..1204
                     /vntifkey="21"
                     /label=3xFlag
                     /note="/vntifkey=21"
     misc_feature    1205..5305
                     /vntifkey="21"
                     /label=eCas91.1
                     /note="/vntifkey=21"
     misc_feature    5306..5326
                     /vntifkey="21"
                     /label=NLS
                     /note="/vntifkey=21"
     misc_feature    5333..5401
                     /vntifkey="21"
                     /label=2A
                     /note="/vntifkey=21"
     misc_feature    5408..6118
                     /vntifkey="21"
                     /label=RFP
                     /note="/vntifkey=21"
     polyA_signal    6174..6398
                     /vntifkey="25"
                     /label=BGH\pA
                     /note="/vntifkey=25"
     misc_feature    6571..7365
                     /vntifkey="21"
                     /label=KanR
                     /note="/vntifkey=21"
     rep_origin      7664..8337
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=pUC\ori
                     /note="/vntifkey=33"
     misc_feature    35..298
                     /vntifkey="21"
                     /label=U6\Promoter
     misc_feature    299..400
                     /vntifkey="21"
                     /label=gRNA
BASE COUNT     2210 a      2202 c      2277 g      1635 t        19 others
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgtaaggtc gggcaggaag agggcctatt 
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat 
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt 
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt 
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgn 
      301 nnnnnnnnnn nnnnnnnngt tttagagcta gaaatagcaa gttaaaataa ggctagtccg 
      361 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt ctaggcccag ctttcttgta 
      421 caaagttggc attagtcgac aagcaagcaa ccttaattaa tgacattgat tattgactag 
      481 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt 
      541 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac 
      601 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg 
      661 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag 
      721 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat 
      781 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat 
      841 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt 
      901 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga 
      961 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg 
     1021 gtgggaggtc tatataagca accaggttct ggctaactag agaacccact gcttactggc 
     1081 ttatcgaaat taatacgact cactataggg agacccaagc tggctagccg ccaccatgga 
     1141 ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga 
     1201 taaggacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt 
     1261 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg 
     1321 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga 
     1381 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg 
     1441 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag 
     1501 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg 
     1561 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa 
     1621 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca 
     1681 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga 
     1741 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc 
     1801 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag 
     1861 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa 
     1921 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga 
     1981 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc 
     2041 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat 
     2101 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc 
     2161 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg 
     2221 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc 
     2281 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct 
     2341 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg 
     2401 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca 
     2461 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat 
     2521 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag 
     2581 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga 
     2641 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa 
     2701 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt 
     2761 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct 
     2821 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac 
     2881 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat 
     2941 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat 
     3001 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt 
     3061 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc 
     3121 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg 
     3181 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct 
     3241 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga 
     3301 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct 
     3361 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac 
     3421 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt 
     3481 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag 
     3541 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc 
     3601 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg 
     3661 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca 
     3721 tatcgtgcct cagagctttc tggcggacga ctccatcgac aacaaggtgc tgaccagaag 
     3781 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa 
     3841 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct 
     3901 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca 
     3961 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa 
     4021 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc 
     4081 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa 
     4141 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa 
     4201 gtaccctgcg ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa 
     4261 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag 
     4321 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaaggc 
     4381 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt 
     4441 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt 
     4501 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat 
     4561 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc 
     4621 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt 
     4681 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga 
     4741 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa 
     4801 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact 
     4861 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag 
     4921 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga 
     4981 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt 
     5041 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa 
     5101 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc 
     5161 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa 
     5221 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat 
     5281 cgacctgtct cagctgggag gcgaccccaa gaaaaagcgc aaagtggtta acggcagtgg 
     5341 agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccacgatc 
     5401 gatgcatgtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat 
     5461 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta 
     5521 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt 
     5581 cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc acacccaggg 
     5641 catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac 
     5701 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct 
     5761 catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa 
     5821 gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgga 
     5881 aggcagaagc gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttcaa 
     5941 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt 
     6001 ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga 
     6061 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac ttaatgctgt 
     6121 taactgatga ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 
     6181 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     6241 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     6301 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     6361 caatagcagg catgctgggg atgcggtggg ctctatggct tctactgggc ggttttatgg 
     6421 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 
     6481 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct 
     6541 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 
     6601 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 
     6661 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 
     6721 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 
     6781 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 
     6841 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 
     6901 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 
     6961 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 
     7021 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 
     7081 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 
     7141 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 
     7201 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 
     7261 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 
     7321 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac 
     7381 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg 
     7441 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     7501 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat agcacgtgct 
     7561 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 
     7621 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 
     7681 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 
     7741 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 
     7801 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 
     7861 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 
     7921 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 
     7981 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 
     8041 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 
     8101 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 
     8161 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 
     8221 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 
     8281 cgccagcaac gcggcctttt tacggttcct gggcttttgc tggccttttg ctcacatgtt 
     8341 ctt 
//
