LOCUS       p57                     9739 bp    DNA     circular     22-AUG-2017
DEFINITION  .
ACCESSION   urn.local...9t-7o8q9kz
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer          63..86
                     /label="Sequencing XhoI Rev primer"
     misc_feature    179..973
                     /label="Kan R"
     primer_bind     426..445
                     /label="TDNA-pUCRev"
     misc_feature    1273..1946
                     /label="pUC Ori"
     primer_bind     1823..1842
                     /label="pUC-THMS2 primer Forw"
     misc_feature    1981..1986
                     /Recognition_pattern="G^TCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    2005..2010
                     /Recognition_pattern="T^CTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    2011..2036
                     /label="T-DNA LB"
     misc_feature    2037..2044
                     /Recognition_pattern="GTTT^AAAC"
                     /label="PmeI"
                     /note="Geneious type: restriction site"
     loci            complement(2045..3160)
                     /label="NPTII"
     misc_feature    3161..3166
                     /Recognition_pattern="G^GTACC"
                     /label="Acc65I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3167..3997)
                     /label="35 S promoter"
     misc_feature    3998..4003
                     /Recognition_pattern="G^AATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     allele          4004..4366
                     /label="Wh-U6"
     misc_feature    4251..4256
                     /Recognition_pattern="G^TCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     attenuator      4371..4377
                     /label="re-AarI"
     attenuator      4386..4392
                     /label="AarI"
     attenuator      4397..4479
                     /label="Mo-sgRNA"
     misc_feature    4480..4485
                     /label="Barcode"
     misc_feature    4486..4491
                     /Recognition_pattern="A^AGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     -35_signal      4520..5300
                     /label="35S promoter"
     misc_feature    5334..5339
                     /Recognition_pattern="G^CTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     C_region        5340..9471
                     /label="ZmCas9"
     misc_feature    7528..7533
                     /Recognition_pattern="G^GATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    9472..9477
                     /Recognition_pattern="C^CCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    9661..9666
                     /Recognition_pattern="AGG^CCT"
                     /label="StuI"
                     /note="Geneious type: restriction site"
     misc_feature    9667..9690
                     /label="T-DNA RB"
     misc_feature    9734..9739
                     /label="XhoI"
ORIGIN      
        1 ctcgagcttc tactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
       61 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct cgccgccaag
      121 gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat
      181 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      241 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      301 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      361 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      421 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      481 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      541 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      601 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      661 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
      721 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
      781 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
      841 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
      901 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
      961 cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     1021 tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt gcgcggaacc
     1081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1141 tgataaatgc ttcaataata gcacgtgcta aaacttcatt tttaatttaa aaggatctag
     1201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1441 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1921 ggcttttgct ggccttttgc tcacatgttc ttgactcttc gcgatgtacg ggccagatat
     1981 gtcgacgtaa aacgacggcc agtgtctaga tggcaggata tattgtggtg taaacagttt
     2041 aaacagtgtt ttactcctca tattaacttc ggtcattaga ggccacgatt tgacacattt
     2101 ttactcaaaa caaaatgttt gcatatctct tataatttca aattcaacac acaacaaata
     2161 agagaaaaaa caaataatat taatttgaga atgaacaaaa ggaccatatc attcattaac
     2221 tcttctccat ccatttccat ttcacagttc gatagcgaaa accgaataaa aaacacagta
     2281 aattacaagc acaacaaatg gtacaagaaa aacagttttc ccaatgccat aatactcgaa
     2341 cgtccggagt tatcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     2401 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     2461 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     2521 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     2581 gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag
     2641 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     2701 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     2761 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     2821 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     2881 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     2941 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt
     3001 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     3061 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     3121 tgcgtgcaat ccatcttgtt caatccaagc tcccattgtt ggtacccagc ttgggtctag
     3181 tcgtattaag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
     3241 gaaatgaact tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc
     3301 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     3361 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     3421 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     3481 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     3541 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     3601 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac
     3661 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     3721 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     3781 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     3841 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     3901 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     3961 ctccaccatt acataggccc atcggagcta acgcagtgaa ttcgaccaag cccgttattc
     4021 tgacagttct ggtgctcaac acatttatat ttatcaagga gcacattgtt actcactgct
     4081 aggagggaat cgaactagga atattgatca gaggaactac gagagagctg aagataactg
     4141 ccctctagct ctcactgatc tgggtcgcat agtgagatgc agcccacgtg agttcagcaa
     4201 cggtctagcg ctgggctttt aggcccgcat gatcgggctt ttgtcgggtg gtcgacgtgt
     4261 tcacgattgg ggagagcaac gcagcagttc ctcttagttt agtcccacct cgcctgtcca
     4321 gcagagttct gaccggttta taaactcgct tgctgcatca gacttggctc gcaggtggaa
     4381 gaatgcacct gcgagagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     4441 atcaacttga aaaagtggca ccgagtcggt gctttttttg tggccaagct tactgcgtta
     4501 gctccgatgg gcctatgtaa tggtggagca cgacactctc gtctactcca agaatatcaa
     4561 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
     4621 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     4681 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     4741 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     4801 agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga
     4861 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     4921 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     4981 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     5041 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     5101 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     5161 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     5221 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct
     5281 ctctctacaa atctatctct taatacgact cactataggg agacccaagc tgggctagca
     5341 acaatggaca agaagtacag catcggcctg gacatcggca ccaactctgt tggctgggct
     5401 gtgatcaccg acgagtacaa ggtgccgagc aagaagttca aggtcctggg caacaccgac
     5461 aggcacagca tcaagaagaa cctgatcggc gccctgctgt tcgactctgg tgagactgct
     5521 gaggccacca ggctgaagag gactgctaga agaaggtaca ccaggcgcaa gaacaggatc
     5581 tgctacctgc aagagatttt cagcaacgag atggccaagg tggacgacag cttcttccac
     5641 aggcttgagg agagcttcct ggtcgaggag gataagaagc acgagaggca cccgatcttc
     5701 ggcaacatcg tggatgaggt ggcctaccac gagaagtacc cgaccatcta ccacctccgc
     5761 aagaagctgg ttgacagcac cgacaaggct gacctgaggc tgatctacct ggccctggcc
     5821 cacatgatca agttcagggg ccacttcctc atcgagggcg acctgaaccc agacaacagc
     5881 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggagaac
     5941 ccgatcaacg cctctggcgt ggacgctaag gctatcctgt ctgccaggct gagcaagagc
     6001 aggcgccttg agaaccttat cgctcagctg ccgggcgaga agaagaacgg ccttttcggc
     6061 aacctgattg ccctgtctct gggcctgacc ccgaacttca agagcaactt cgacctggcc
     6121 gaggacgcca agctccagct gtctaaggac acctacgacg acgacctgga caacctgctt
     6181 gctcagatcg gcgaccagta cgccgacctg ttcctggctg ctaagaacct gtctgacgcc
     6241 atcctgctga gcgacatcct gagggtgaac accgagatca ccaaggctcc actgagcgcc
     6301 tctatgatca agcgctacga cgagcaccac caggacctga ctctgctgaa ggctctggtt
     6361 aggcagcagc tgccagagaa gtacaaggag attttcttcg accagagcaa gaacggctac
     6421 gccggctaca ttgatggcgg cgcttctcaa gaggagttct acaagttcat caagccgatc
     6481 cttgagaaga tggacggcac cgaggagctg ctggttaagc tgaacagaga ggacctgctg
     6541 aggaagcaga ggaccttcga caacggcagc atcccgcacc agatccatct gggcgagctg
     6601 catgctatcc tgaggcgcca agaggacttc tacccgttcc tgaaggacaa ccgcgagaag
     6661 attgagaaga tcctgacctt ccgcatcccg tactacgttg gcccacttgc taggggcaac
     6721 agcaggttcg cttggatgac ccgcaagagc gaggagacta tcaccccctg gaacttcgag
     6781 gaggtggtgg acaagggcgc ttctgcccag tctttcatcg agaggatgac caacttcgat
     6841 aagaacctcc cgaacgagaa ggtgctgccg aagcactctc tgctgtacga gtacttcacc
     6901 gtgtacaacg agctgacgaa ggtcaagtac gtgaccgagg gtatgaggaa gccagccttc
     6961 ctgagcggcg agcaaaagaa ggccatcgtc gatctgctgt tcaagaccaa ccgcaaggtg
     7021 accgtgaagc agctcaagga ggactacttc aagaagatcg agtgcttcga ctccgtcgag
     7081 atcagcggcg ttgaggacag gttcaacgct tccctgggca cctaccatga cctgctcaag
     7141 attatcaagg acaaggactt cctcgacaac gaggagaatg aggacattct tgaggacatc
     7201 gtgctgaccc tgaccctctt cgaggaccgc gagatgatcg aggagaggct caagacctac
     7261 gcccacctct tcgacgacaa ggtgatgaag caactgaaga ggcgcaggta cactggctgg
     7321 ggcaggcttt ctaggaagct gatcaacggc atcagggaca agcagagcgg caagacgatc
     7381 ctcgacttcc tgaagtctga cggcttcgct aaccgcaact tcatgcagct gatccacgac
     7441 gacagcctca ccttcaagga ggatatccag aaggcccagg ttagcggcca gggcgattct
     7501 ctgcatgagc atatcgctaa cctcgccgga tccccggcta tcaagaaggg catcctgcag
     7561 accgtcaaga tcgtggacga gctggtgaag gtgatgggcc acaagccaga gaacatcgtg
     7621 atcgagatgg cgcgcgagaa ccagaccact cagaagggcc aaaagaacag ccgcgagcgc
     7681 atgaagagga ttgaggaggg cattaaggag ctgggcagcc agatcctcaa ggagcaccca
     7741 gttgagaaca cccagctgca gaatgagaag ctgtacctgt actacctgca gaacggcagg
     7801 gacatgtacg tggaccaaga gctggacatc aacaggctgt ccgactacga cgttgaccac
     7861 atcgtgccgc agagcttcat caaggacgac tccatcgaca acaaggtcct cacccgcagc
     7921 gacaagaacc gcggcaagtc tgacaacgtg ccaagcgagg aggtcgtgaa gaagatgaag
     7981 aactactggc ggcagctcct gaacgcgaag ctcatcaccc agcgcaagtt cgacaacctg
     8041 accaaggctg agagaggcgg cctgagcgag cttgataagg ccggcttcat taagaggcag
     8101 ctggtcgaga ctaggcagat cacgaagcac gtggcccaga tcctggacag ccgcatgaac
     8161 accaagtacg acgagaacga caagctgatc cgcgaggtga aggttatcac cctgaagtcc
     8221 aagctggtga gcgacttccg caaggatttc caattctaca aggtccgcga gatcaacaac
     8281 taccaccacg cccacgacgc ctacctgaac gctgttgttg gcaccgccct gatcaagaag
     8341 tacccaaagt tggagagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcgcaag
     8401 atgatcgcca agtcggagca agagatcggc aaggcgaccg ccaagtactt cttctacagc
     8461 aacatcatga atttcttcaa gactgagatc accctggcca acggcgagat cagaaagagg
     8521 ccgctcattg agactaatgg cgagactggc gagatcgtgt gggataaggg cagggacttc
     8581 gccactgtgc gcaaggtgct gtctatgcca caggtgaaca tcgttaagaa gaccgaggtc
     8641 cagaccggcg gcttcagcaa ggagtctatc ctgccgaaga ggaacagcga taagctgatc
     8701 gcccggaaga aggactggga cccaaagaag tacggcgggt tcgatagccc aaccgtggct
     8761 tactctgtgc tggtggtggc gaaggtcgag aagggcaagt ccaagaagct caagagcgtc
     8821 aaggagcttc tgggcatcac catcatggag aggtccagct tcgagaagaa tccaatcgat
     8881 ttccttgagg ccaagggcta caaggaggtc aagaaggacc tcatcatcaa gctgccaaag
     8941 tactccctgt tcgagttgga gaacggccgc aagaggatgc tggcttctgc tggcgagctt
     9001 caaaagggca acgagcttgc cctgccgtcc aagtacgtca acttcctgta cctggccagc
     9061 cactacgaga agctgaaggg ctctccagag gacaatgagc aaaagcagct tttcgtcgag
     9121 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttcag caagagggtg
     9181 atcctggccg acgccaacct ggataaggtg ctcagcgcct acaacaagca ccgcgacaag
     9241 ccaattcgcg agcaggccga gaacatcatc cacctgttca cgctgaccaa cctgggtgcc
     9301 ccagcagcct tcaagtactt cgataccacc atcgaccgca agcggtacac ctccactaag
     9361 gaggttctgg acgccaccct gatccaccag tctatcactg gcctgtacga gacaaggatc
     9421 gacctctcac agctgggcgg cgaccctaag aagaagagga aggtttgatg acccgggtct
     9481 ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct
     9541 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     9601 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta
     9661 aggccttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     9721 cggatattta aaactcgag
//
