LOCUS       p61                     9418 bp    DNA     circular     22-AUG-2017
DEFINITION  .
ACCESSION   urn.local...94-7o8q7k5
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer          63..86
                     /label="Sequencing XhoI Rev primer"
     misc_feature    179..973
                     /label="Kan R"
     primer          426..445
                     /label="TDNA-pU Rev"
     misc_feature    739..744
                     /Recognition_pattern="C^CATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    1273..1946
                     /label="pUC Ori"
     primer          1823..1842
                     /label="pUC-THMS-F"
     primer          1901..1924
                     /label="P21"
     misc_feature    1981..1986
                     /Recognition_pattern="G^TCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    1993..1998
                     /Recognition_pattern="T^CTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    1999..2024
                     /label="T-DNA LB"
     misc_feature    2025..2032
                     /Recognition_pattern="GTTT^AAAC"
                     /label="PmeI"
                     /note="Geneious type: restriction site"
     loci            complement(2033..2890)
                     /label="BAR"
     primer          2051..2072
                     /label="BAR-F"
     primer          2208..2227
                     /label="BarTerm-R"
     misc_feature    2324..2331
                     /Recognition_pattern="GTTT^AAAC"
                     /label="PmeI"
                     /note="Geneious type: restriction site"
     primer          2549..2568
                     /label="BAR-R"
     primer          2701..2721
                     /label="BarTerm-F"
     misc_feature    2891..2896
                     /Recognition_pattern="G^GTACC"
                     /label="Acc65I"
                     /note="Geneious type: restriction site"
     misc_feature    2891..2896
                     /Recognition_pattern="GGTAC^C"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2897..3727)
                     /label="35 S promoter"
     misc_feature    3728..3733
                     /Recognition_pattern="G^AATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     allele          3734..4038
                     /label="AtU6"
     misc_feature    3924..3929
                     /Recognition_pattern="AGG^CCT"
                     /label="StuI"
                     /note="Geneious type: restriction site"
     misc_feature    4021..4043
                     /label="AtU6R1"
     misc_feature    complement(4043..4049)
                     /Recognition_pattern="CACCTGC(4/8)"
                     /label="AarI"
                     /note="Geneious type: restriction site"
     misc_feature    4060..4066
                     /Recognition_pattern="CACCTGC(4/8)"
                     /label="AarI"
                     /note="Geneious type: restriction site"
     S_region        4071..4153
                     /label="sgRNA scaffold"
     misc_feature    4154..4159
                     /label="Barcode"
     misc_feature    4160..4165
                     /Recognition_pattern="A^AGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     -35_signal      4194..4974
                     /label="35S promoter"
     misc_feature    5007..5012
                     /Recognition_pattern="G^CTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     allele          5013..9150
                     /label="At-Cas9"
     misc_feature    7201..7206
                     /Recognition_pattern="G^GATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     insertion       7202..9342
                     /label="Insert 1"
     misc_feature    9139..9144
                     /Recognition_pattern="A^GATCT"
                     /label="BglII"
                     /note="Geneious type: restriction site"
     misc_feature    9151..9156
                     /Recognition_pattern="C^CCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    9340..9345
                     /Recognition_pattern="AGG^CCT"
                     /label="StuI"
                     /note="Geneious type: restriction site"
     misc_feature    9346..9369
                     /label="T-DNA RB"
     misc_feature    9413..9418
                     /label="XhoI"
ORIGIN      
        1 ctcgagcttc tactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
       61 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct cgccgccaag
      121 gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat
      181 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      241 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      301 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      361 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      421 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      481 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      541 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      601 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      661 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
      721 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
      781 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
      841 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
      901 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
      961 cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     1021 tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt gcgcggaacc
     1081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1141 tgataaatgc ttcaataata gcacgtgcta aaacttcatt tttaatttaa aaggatctag
     1201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1441 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1921 ggcttttgct ggccttttgc tcacatgttc ttgactcttc gcgatgtacg ggccagatat
     1981 gtcgacgatg tatctagatg gcaggatata ttgtggtgta aacagtttaa acagtgtttt
     2041 actcctcata ttaacttcgg tcattagagg ccacgatttg acacattttt actcaaaaca
     2101 aaatgtttgc atatctctta taatttcaaa ttcaacacac aacaaataag agaaaaaaca
     2161 aataatatta atttgagaat gaacaaaagg accatatcat tcattaactc ttctccatcc
     2221 atttccattt cacagttcga tagcgaaaac cgaataaaaa acacagtaaa ttacaagcac
     2281 aacaaatggt acaagaaaaa cagttttccc aatgccataa tacgtttaaa cttatcagat
     2341 ttcggtgacg ggcaggaccg gacggggcgg caccggcagg ctgaagtcca gctgccagaa
     2401 acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc
     2461 atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac
     2521 cacgctcttg aagccctgtg cctccaggga cttcaggagg tgggtgtaga gcgtggagcc
     2581 cagtcccgtc cgctggtggc ggggggagac gtacacggtg gactcggccg tccagtcgta
     2641 ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc
     2701 ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg
     2761 ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt
     2821 gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg
     2881 gcccattgtt ggtacccagc ttgggtctag tcgtattaag agatagattt gtagagagag
     2941 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaaggtc
     3001 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
     3061 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     3121 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
     3181 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
     3241 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     3301 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     3361 gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     3421 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     3481 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     3541 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     3601 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     3661 gatattcttg gagtagacga gagtgtcgtg ctccaccatt acataggccc atcggagcta
     3721 acgcagtgaa ttcagaaatc tcaaaattcc ggcagaacaa ttttgaatct cgatccgtag
     3781 aaacgagacg gtcattgttt tagttccacc acgattatat ttgaaattta cgtgagtgtg
     3841 agtgagactt gcataagaaa ataaaatctt tagttgggaa aaaattcaat aatataaatg
     3901 ggcttgagaa ggaagcgagg gataggcctt tttctaaaat aggcccattt aagctattaa
     3961 caatcttcaa aagtaccaca gcgcttaggt aaagaaagca gctgagttta tatatggtta
     4021 gagacgaagt agtgattgga tggcaggtgg aagaatggac acctgcgaga gttttagagc
     4081 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     4141 cggtgctttt tttgtttcga agcttactgc gttagctccg atgggcctat gtaatggtgg
     4201 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
     4261 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     4321 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
     4381 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
     4441 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     4501 aagtggattg atgtgataac atggtggagc acgacactct cgtctactcc aagaatatca
     4561 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
     4621 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
     4681 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     4741 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     4801 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     4861 gggatgacgc acaatcccac tatccttcgc aagaccttcc tctatataag gaagttcatt
     4921 tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat ctcttaatac
     4981 gactcactat agggagaccc aagctggcta gcaacaatgg ataagaagta ctctatcgga
     5041 ctcgatatcg gaactaactc tgttggatgg gctgtgatca ccgatgagta caaggtgcca
     5101 tctaagaagt tcaaggttct cggaaacacc gataggcact ctatcaagaa aaaccttatc
     5161 ggtgctctcc tcttcgattc tggtgaaact gctgaggcta ccagactcaa gagaaccgct
     5221 agaagaaggt acaccagaag aaagaacagg atctgctacc tccaagagat tttctctaac
     5281 gagatggcta aagtggatga ttcattcttc cacaggctcg aagagtcatt cctcgtggaa
     5341 gaagataaga agcacgagag gcaccctatc ttcggaaaca tcgttgatga ggtggcatac
     5401 cacgagaagt accctactat ctaccacctc agaaagaagc tcgttgattc tactgataag
     5461 gctgatctca ggctcatcta cctcgctctc gctcacatga tcaagttcag aggacacttc
     5521 ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg ataagttgtt catccagctc
     5581 gtgcagacct acaaccagct tttcgaagag aaccctatca acgcttcagg tgtggatgct
     5641 aaggctatcc tctctgctag gctctctaag tcaagaaggc ttgagaacct cattgctcag
     5701 ctccctggtg agaagaagaa cggacttttc ggaaacttga tcgctctctc tctcggactc
     5761 acccctaact tcaagtctaa cttcgatctc gctgaggatg caaagctcca gctctcaaag
     5821 gatacctacg atgatgatct cgataacctc ctcgctcaga tcggagatca gtacgctgat
     5881 ttgttcctcg ctgctaagaa cctctctgat gctatcctcc tcagtgatat cctcagggtg
     5941 aacaccgaga tcaccaaggc tccactttct gcttctatga tcaagagata cgatgagcac
     6001 caccaggatc tcacacttct caaggctctt gttagacagc agctcccaga gaagtacaaa
     6061 gaaatcttct tcgatcagtc taagaacgga tacgctggtt acatcgatgg tggtgcatct
     6121 caagaagagt tctacaagtt catcaagcca atcttggaga agatggatgg aaccgaggaa
     6181 ctcctcgtga agctcaatag agaggatctc cttaggaagc agaggacctt cgataacgga
     6241 tctatccctc atcagatcca cctcggagag ttgcacgcta tccttagaag gcaagaggat
     6301 ttctacccat tcctcaagga taacagagag aagattgaga agatcctcac cttcagaatc
     6361 ccttactacg tgggacctct cgctagagga aactcaagat tcgcttggat gaccagaaag
     6421 tctgaggaaa ccatcacccc ttggaacttc gaagaggtgg tggataaggg tgctagtgct
     6481 cagtctttca tcgagaggat gaccaacttc gataagaacc ttcctaacga gaaggtgctc
     6541 cctaagcact ctttgctcta cgagtacttc accgtgtaca acgagttgac caaggttaag
     6601 tacgtgaccg agggaatgag gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc
     6661 gttgatctct tgttcaagac caacagaaag gtgaccgtga agcagctcaa agaggattac
     6721 ttcaagaaaa tcgagtgctt cgattcagtg gaaatctctg gtgttgagga taggttcaac
     6781 gcatctctcg gaacctacca cgatctcctc aagatcatta aggataagga tttcttggat
     6841 aacgaggaaa acgaggatat cttggaggat atcgttctta ccctcaccct cttcgaggat
     6901 agagagatga tagaagaaag gctcaagacc tacgctcatc tcttcgatga taaggtgatg
     6961 aagcagttga agagaagaag atacactggt tggggaaggc tctcaagaaa gctcattaac
     7021 ggaatcaggg ataagcagtc tggaaagaca atccttgatt tcctcaagtc tgatggattc
     7081 gctaacagaa acttcatgca gctcatccac gatgattctc tcacctttaa agaggatatc
     7141 cagaaggctc aggtttcagg acagggtgat agtctccatg agcatatcgc taacctcgct
     7201 ggatcccctg caatcaagaa gggaatcctc cagactgtga agattgtgga tgagttggtg
     7261 aaggtgatgg gacacaagcc tgagaacatc gtgatcgaaa tggctagaga gaaccagacc
     7321 actcagaagg gacagaagaa ctctagggaa aggatgaaga ggatcgagga aggtatcaaa
     7381 gagcttggat ctcagatcct caaagagcac cctgttgaga acactcagct ccagaacgag
     7441 aagctctacc tctactactt gcagaacgga agggatatgt atgtggatca agagcttgat
     7501 attaacaggc tctctgatta cgatgttgat catatcgtgc cacagtcttt tatcaaagat
     7561 gattctatcg ataacaaggt gctcactagg tctgataaga acaggggtaa gagtgataac
     7621 gtgccaagtg aagaggttgt gaagaaaatg aagaactatt ggaggcagct cctcaacgct
     7681 aagctcatca ctcagagaaa gttcgataac ttgaccaagg ctgagagggg aggactctct
     7741 gaattggata aggcaggatt catcaagaga cagctcgtgg aaaccaggca gatcaccaaa
     7801 catgtggcac agatcctcga ttctaggatg aacaccaagt acgatgagaa cgataagttg
     7861 atcagggaag tgaaggttat caccctcaag tcaaagctcg tgtctgattt cagaaaggat
     7921 ttccaattct acaaggtgag ggaaatcaac aactaccacc acgctcacga tgcttacctt
     7981 aacgctgttg ttggaaccgc tctcatcaag aagtatccaa agttggagtc tgagttcgtg
     8041 tacggtgatt ataaggtgta cgatgtgagg aagatgatcg ctaagtctga gcaagagatc
     8101 ggaaaggcta ccgctaagta tttcttctac tctaacatca tgaatttctt caagaccgag
     8161 atcactctcg ctaacggtga gatcagaaag aggccactca tcgagacaaa cggtgaaaca
     8221 ggtgagatcg tgtgggataa gggaagggat ttcgctaccg ttagaaaggt gctctctatg
     8281 cctcaggtga acatcgttaa gaaaaccgag gtgcagaccg gtggattctc taaagagtct
     8341 atcctcccta agaggaactc tgataagctc attgctagga agaaggattg ggaccctaag
     8401 aaatacggtg gtttcgattc tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt
     8461 gagaagggaa agagtaagaa gctcaagtct gttaaggaac ttctcggaat cactatcatg
     8521 gaaaggtcat ctttcgagaa gaacccaatc gatttccttg aggctaaggg atacaaagag
     8581 gttaagaagg atctcatcat caagctccca aagtactcac ttttcgagtt ggagaacggt
     8641 agaaagagga tgctcgcttc tgctggtgag cttcaaaagg gaaacgagct tgctctccca
     8701 tctaagtacg ttaactttct ttacctcgct tctcactacg agaagttgaa gggatctcca
     8761 gaagataacg agcagaagca acttttcgtt gagcagcaca agcactactt ggatgagatc
     8821 atcgagcaga tcagtgagtt ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag
     8881 gtgttgtctg cttacaacaa gcacagagat aagcctatca gggaacaggc agagaacatc
     8941 atccatctct tcacccttac caacctcggt gctcctgctg ctttcaagta cttcgataca
     9001 accatcgata ggaagagata cacctctacc aaagaagtgc tcgatgctac cctcatccat
     9061 cagtctatca ctggactcta cgagactagg atcgatctct cacagcttgg aggtgatcct
     9121 aagaagaaaa gaaaggttag atcttgatga cccgggtctc cataataatg tgtgagtagt
     9181 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     9241 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     9301 aaccaaaatc cagtactaaa atccagatcc cccgaattaa ggccttgaca ggatatattg
     9361 gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa aactcgag
//
