Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the HDAC2-62 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human histone deacetylase 2 (amino acid residues with N-terminal added cysteine) conjugated to maleimide-activated KLH. Histone deacetylase 2 (HDAC2) is the catalytic subunits of different multiprotein regulatory complexes.

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal has been used in:

• co-immunoprecipitation
• chromatin immunoprecipitation (ChIP)
• western blot
• immunohistochemistry
• enzyme linked immunosorbent assays (ELISA)

Histone Deacetylases including HDAC′s 1-10 facilitate transcriptional repression and nucleosome condensation. Abnormal recruitment of HDACs by various oncoproteins may occur in certain neoplastic diseases. Inhibition of HDAC2 activity by valproic acid induces proteasomal degradation of HDAC2.

Monoclonal Anti-Histone Deacetylase 2 (HDAC2) recognizes human, bovine, dog, rat, mouse, and chicken HDAC2 (approx. 55 kDa). The antibody epitope resides within amino acids 471-488 of human HDAC2.

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

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