跳转至内容
Merck
CN

主要文件

查看所有文档

H9286

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-H3K9ac

登录 查看组织和合同定价。

选择尺寸

变更视图

关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02685921
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB
Citations:
26
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~17 kDa

species reactivity

bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken

concentration

2-2.5 mg/mL

technique(s)

indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate, microarray: suitable, western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

UniProt accession no.

P68431

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys9)

Gene Information

human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)

General description

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for:
  • indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
  • microarray
  • western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

Immunogen

synthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Application

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in:
  • indirect immunofluorescence
  • immunoblotting
  • immunohistochemistry

Biochem/physiol Actions

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.
In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Still not finding the right product?

试用我们的 产品选型工具 工具缩小选择范围

存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料
常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
111M4857
089K4796
072K4824

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Jamie Benoit et al.
Brain structure & function, 221(8), 3963-3978 (2015-11-04)
Histone acetylation is considered a major epigenetic process that affects brain development and synaptic plasticity, as well as learning and memory. The transcriptional effectors and morphological changes responsible for plasticity as a result of long-term modifications to histone acetylation are
Genliang Li et al.
Cytotechnology, 69(1), 75-87 (2016-11-30)
As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding
Gang Xi et al.
Molecular endocrinology (Baltimore, Md.), 22(9), 2162-2175 (2008-07-09)
Our previous studies have indicated an essential role of p52shc in mediating IGF-I activation of MAPK in smooth muscle cells (SMC). However, the role of the p66 isoform of shc in IGF-I signal transduction is unclear. In the current study
查看所有相关文献

全球贸易项目编号

货号GTIN
H9286-200UL04061837794513