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Merck
CN

MAB3484

Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4

clone L12B4, Chemicon®, from mouse

别名:

CFTR

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
L12B4, monoclonal
Application:
ICC, IP, WB
Citations:
19
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

L12B4, monoclonal

species reactivity

human

should not react with

shark, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CFTR(1080)

Immunogen

Epitope: a.a. 386-412 of human CFTR

Application

Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4 is an antibody against Cystic Fibrosis Transmembrane Conductance Regulator for use in IC, IP & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10μg/mL, note do not boil the lysate, incubate at 80C for 30 minutes prior to running SDS-PAGE. CTFR aggregates upon boiling. Antibody recognizes CFTR at 170kDa and two additional proteins at 100 & 140kDa.

Immunoprecipitation

Immunofluorescence

Note: Does not work on paraffin embedded tissue.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Human cystic fibrosis transmembrane conductance regulator (CFTR). Recognizes an epitope at the cytoplasmic region preceding the first nucleotide binding domain between residues 386 to 412.

Recognizes CFTR, Mr 170kDa and two additional proteins at ~ Mr 100 and 140kDa.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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商品

16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.


Lacking CD56 expression in a relapsing cutaneous blastic plasmacytoid dendritic cell neoplasm after allogeneic bone marrow transplantation: FISH analysis revealed loss of 11q.
Mitteldorf C, Bertsch HP, Baumgart M, Haase D, Wulf G, Schon MP, Rosenwald A, Neumann C, Kaune KM.
Journal of the European Academy of Dermatology and Venereology : JEADV null
Targeted quantitation of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple reaction monitoring tandem mass spectrometry and oxygen stable isotope dilution.
Jiang H, Ramos AA, Yao X
Analytical Chemistry null
Mauricio Di Fulvio et al.
PloS one, 15(12), e0242749-e0242749 (2020-12-03)
Cystic fibrosis (CF) is due to mutations in the CF-transmembrane conductance regulator (CFTR) and CF-related diabetes (CFRD) is its most common co-morbidity, affecting ~50% of all CF patients, significantly influencing pulmonary function and longevity. Yet, the complex pathogenesis of CFRD



全球贸易项目编号

货号GTIN
MAB348404053252502392