description
non-ionic
form
viscous liquid
mol wt
~603
CMC
0.08 mM (20-25°C)
viscosity
250-350 cP, neat(25 °C)(lit.)
density
1.06 g/cm3 at 25 °C (lit.)
HLB
13
SMILES string
O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C
InChI
1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3
InChI key
JYCQQPHGFMYQCF-UHFFFAOYSA-N
General description
IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.
Application
IGEPAL® CA-630 has been used:
- as a component of grinding buffer along with lithium dodecyl sulphate, tris-HCl, EDTA, NaCl, sodium deoxycholate, dithiothreitol and thiourea in RNA extraction process
- along with Tris- HCl in incubation of Tachyzoites cultured in vero cells
- as a component of nuclear extract buffer D+ containing HEPES, glycerol, KCl and EDTA for gel shift analysis
- as a component of PI buffer along with NaH2PO4 to wash slides in FISH (fluorescence in situ hybridization) procedure
- for solubilizing and isolation of membrane proteins
Analysis Note
Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.
Other Notes
Nonionic, non-denaturing detergent
Legal Information
IGEPAL is a registered trademark of Solvay
Disclaimer
May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.
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signalword
Danger
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
483.8 °F - closed cup
flash_point_c
251 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
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Protocols
This page shows how to solubilize membrane proteins with products from Cytiva.
Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize.
Kato A, et al.
Proceedings of the National Academy of Sciences of the USA, 101(37), 13554-13559 (2004)
Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay.
Phytopathology, 90(5), 448-453 (2000)
Automated time-resolved immunofluorometric assay for Toxoplasma gondii-specific IgM and IgA antibodies: study of more than 130 000 filter-paper blood-spot samples from newborns.
Clinical Chemistry, 48(11), 1981-1986 (2002)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| I3021-100ML | 04061837596858 |
| I3021-500ML | 04061833848814 |
| I3021-50ML | 04061833848821 |
| I3021-5ML | 04061837596889 |