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Calcium Phosphate Transfection Kit

Name: Calcium Phosphate Transfection Kit
Brand: SIGMA

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

Synonym(s):

Gene delivery

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About This Item

UNSPSC Code:

12352200

EC Number:

233-140-8

NACRES:

NA.85

Form:

solution

Grade:

Molecular Biology

Technique(s):

transfection: suitable

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Properties

grade

Molecular Biology

form

solution

usage

kit sufficient for 160 transfections (6 cm dishes), kit sufficient for 400 transfections (3.5 cm dishes), kit sufficient for 80 transfections (10 cm dishes)

technique(s)

transfection: suitable

shipped in

dry ice

storage temp.

−20°C

Description

General description

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.

Application

Calcium Phosphate Transfection Kit has been used:

to enable transfection
to transfect Hek293T cells
to transfect H29D cells

Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:

BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D

Biochem/physiol Actions

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl₂ solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.

Features and Benefits

Suitable for transient and stable transfection
Reproducible for a wide range of cell types
Widely referenced
Inexpensive

Other Notes

The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)

The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 1

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Protocols

磷酸钙转染试剂盒实验方案

使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Articles

细胞转染简介

转染是将DNA、RNA或蛋白质引入真核细胞的过程，用于研究和调节基因表达。因此，转染技术和实验方案作为分析工具，有助于表征遗传功能、蛋白质合成、细胞生长和发育。

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Global Trade Item Number

SKU	GTIN
CAPHOS-1KT	04061833531464