D0547
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME/F-12, 1:1 mixture
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About This Item
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:
- to culture cerebral cortices from mice embryos
- as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
- to culture human corneal epithelial cell lines
Preparation Note
Formulated to contain 12.0 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate
Other Notes
Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate
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Protocols
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
Nutrient Mixture F-12 Ham Formulation
Christo J Botha et al.
The Onderstepoort journal of veterinary research, 87(1), e1-e4 (2020-04-04)
Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis
Selma M Soyal et al.
Molecular neurobiology, 57(2), 752-764 (2019-09-01)
PPARGC1A encodes a transcriptional co-activator also termed peroxisome proliferator-activated receptor (PPAR) gamma coactivator 1-alpha (PGC-1α) which orchestrates multiple transcriptional programs. We have recently identified CNS-specific transcripts that are initiated far upstream of the reference gene (RG) promoter. The regulation of
Ceyda Acilan et al.
Biochimica et biophysica acta, 1861(2), 218-234 (2016-10-25)
To overcome the hurdles of cisplatin, majorly its toxicity and resistance, there has been extensive search for alternative anti-cancer metal-based compounds. Here, three Cu(II)-complexes, Cu(Sal-Gly)(phen), Cu(Sal-Gly)(pheamine), Cu(Sal-Gly)(phepoxy) are characterized for their interaction with DNA, cytotoxicity and mechanism of action. The
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D0547-25KG | 04061834408246 |
| D0547-10X1L | 04061833557853 |
| D0547-50KG | 04061834408253 |
| D0547-10L | 04061833557846 |