P2783
Phosphotransacetylase from Bacillus stearothermophilus
lyophilized powder, ≥3,000 units/mg protein (biuret)
Synonym(s):
Acetyl-CoA:orthophosphate acetyltransferase, Phosphate acetyltransferase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-855-2
MDL number:
Specific activity:
≥3,000 units/mg protein (biuret)
form
lyophilized powder
Quality Level
specific activity
≥3,000 units/mg protein (biuret)
composition
Protein, ≥20%
shipped in
wet ice
storage temp.
−20°C
Application
Phosphotransacetylase, from Bacillus stearothermophilus, is used to convert CoA to acetyl CoA. Phosphotransacetylase (pta) is used to study transport systems for acetate. It is used to study metabolic pathways in various bacterium.
Biochem/physiol Actions
Phosphotransacetylase converts CoA to acetyl CoA.
Physical form
Lyophilized powder containing potassium phosphate
Other Notes
One unit will convert 1.0 μmole of CoA to acetyl CoA per min at pH 7.5 at 30 °C using acetyl phosphate as substrate.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Articles
Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.
Related Content
G D Sprott et al.
Applied and environmental microbiology, 59(4), 1092-1098 (1993-04-01)
Eleven strains of methanogenic bacteria were divided into two groups on the basis of the directionality (oxidative or reductive) of their citric acid pathways. These pathways were readily identified for most methanogens from the patterns of carbon atom labeling in
Mutants of Bacillus stearothermophilus defective in the uptake and metabolism of acetate
Philip R. Mallinder and Anne Moir
Microbiology, 137, 779-785 (1991)
Jing Huang et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 51(4), 480-487 (2011-07-30)
To study the effects of gene pta disruption on biosynthesis of L-tryptophan. The pta gene of the L-tryptophan producing strain E. coli TRTH was disrupted by Red recombination technology and a pta mutant E. coli TRTHdeltapta was constructed. Fed-batch fermentation
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P2783-5KU | 04061832925110 |