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100500C

Avanti

E. coli Extract Total

Avanti Research™ - A Croda Brand

Synonym(s):

E. coli Total Lipid Extract

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About This Item

CAS Number:
1240502-50-4
MDL number:
MFCD18427707
NACRES:
NA.25
UNSPSC Code:
12352211
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assay

>99% (TLC)

form

chloroform solution

packaging

pkg of 2 × 20 mL (100500C-1g), pkg of 1 × 4 mL (100500C-100mg), pkg of 5 × 4 mL (100500C-500mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand

concentration

25 mg/mL (100500C-100mg), 25 mg/mL (100500C-1g), 25 mg/mL (100500C-500mg)

lipid type

lipid extracts

shipped in

dry ice

storage temp.

−20°C

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Application

E. coli Extract Total is suitable:
  • for solubilization of His6-tagged wtABCG2 and its steroid-binding element (SBE) mutant variants
  • to prepare E. coli liposomes for liposomal content release assay
  • for the determination of the phospholipid types bound to purified AdiC in DDM (n-dodecyl-β-D-maltopyranoside) and OM (n-octyl-β-D-maltopyranoside)

E. coli extract total has been used for the preparation of liposomes. It has also been used to characterize the ternary complexes obtained after purification of membrane proteins in different detergents.
E. coli Extract Total has been used:
  • in the preparation of small unilamellar vesicles (SUVs)
  • as a reference standard in electrospray ionization tandem mass spectrometry (ESI-MS/MS)
  • in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies

Biochem/physiol Actions

The E.coli lipid extract contains phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in the identification and taxonomic grouping of any bacterial sample. It is also used for preparing supported lipid bilayers.

Packaging

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100500C-1g)
5 mL Clear Glass Sealed Ampule (100500C-100mg)
5 mL Clear Glass Sealed Ampule (100500C-500mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

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pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

wgk

WGK 3

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

危险化学品
易制毒化学品（2类）
动植物源性产品

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Variation of the Detergent-Binding Capacity and Phospholipid Content of Membrane Proteins When Purified in Different Detergents
Ilgu H, et al.
Biophysical Journal, 106, 1660-1670 (2014)
GhoT of the GhoT/GhoS toxin/antitoxin system damages lipid membranes by forming transient pores
Kim JS, et al.
Biochemical and biophysical research communications, 467-472 (2018)
In vitro systems for Atg8 lipidation
Zens B, et al.
Methods, 75, 37-43 (2015)
View All Related Papers

Global Trade Item Number

SKUGTIN
100500C-100MG04061838168955
100500C-1G04061838168962
100500C-500MG04061838168979