07-452
Anti-dimethyl-Histone H3 (Lys27) Antibody
Upstate®, from rabbit
Synonym(s):
H3K27me2, Histone H3 (di methyl K27)
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
ChIP, DB, WB, cell based assay, multiplexing
Citations:
154
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq), cell based assay: suitable, dot blot: suitable, multiplexing: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
dimethylation (Lys27)
Gene Information
human ... H3C1(8350)
General description
17 kDa
Anti-dimethyl-Histone H3 (Lys27)
Immunogen
KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3
Application
Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)
Dot Blot, Immunocytochemistry (cells)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).
Biochem/physiol Actions
Broad species cross-reactivity expected, based on sequence homology
Recognizes dimethylated histone H3 (Lys27).
Physical form
Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.
Peptide affinity purified
Preparation Note
Stable for 2 years at 2-8°C from date of shipment.
Analysis Note
Control
HeLa whole cell lysate, U2OS cell lysate
HeLa whole cell lysate, U2OS cell lysate
Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The Nucleolus in the Mouse Oocyte is Required for the Early Step of Both Female and Male Pronucleus Organization.
Ogushi S, Saitou M
Journal of Reproduction and Development null
Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes.
Silva, Jose, et al.
Developmental Cell, 4, 481-495 (2003)
Molly Gale et al.
Oncotarget, 7(26), 39931-39944 (2016-10-26)
Lysine demethylase 5A (KDM5A/RBP2/JARID1A) is a histone lysine demethylase that is overexpressed in several human cancers including lung, gastric, breast and liver cancers. It plays key roles in important cancer processes including tumorigenesis, metastasis, and drug tolerance, making it a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 07-452 | 04053252404467 |