Anti-Sp1 Antibody

Sp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.

Varies

Full length human Sp1 protein.

Immunoprecipitation:
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com

Chromatin Immunoprecipitation:
Reported by an independent laboratory.

Immunofluorescence:
Reported by an independent laboratory.

Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.

Expected to cross-react with rat.

Recognizes Sp1, Mr 105 kDa and 95 kDa.

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.

Stable for 1 year at 2-8ºC from date of receipt.

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABE550

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