1.14894
Fractogel® EMD SE Hicap (M)
strong cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
Synonym(s):
Fractogel® EMD SE Hicap (M)
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About This Item
UNSPSC Code:
41115711
ligand
(Sulfoethyl)
Quality Level
product line
Fractogel®
form
resin
specific analyte(s)
MAbs
parameter
220 cm/hr flow rate, 8 bar max. pressure
matrix active group
methacrylate
mean particle size
40-90 μm
capacity
160 mg binding capacity (lysozyme/ml of resin)
transition temp
flash point 35 °C (calculated)
application(s)
recombinant protein
vaccine development
separation technique
cation exchange, strong cation exchange
storage temp.
2-30°C
General description
Strong cation exchange Fractogel® resin featuring the tentacle technology, for purification of basic and neutral proteins (e.g. antibodies) and peptides
Features and Benefits
Fractogel® EMD SE Hicap (M) features include:
- Tentacle technology provides high yield, selectivity, and focused elution
- High loading capacity combined with high purity at lower elution volumes
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Packaging
- 1.14894.0100: Fractogel® EMD SE Hicap (M) Resin 100ml
- 1.14894.0010: Fractogel® EMD SE Hicap (M) Resin 10ml
- 1.14894.0500: Fractogel® EMD SE Hicap (M) Resin 500ml
- 1.14894.5000: Fractogel® EMD SE Hicap (M) Resin 5L
Plastic bottle
Analysis Note
Appearance: Milky turbid suspension,free from impurities(foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.08 %
Cerium: ≤ 20 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 120 - 160 mg/ml
Functional test (b:a): ≤ 0.20
Functional test: Separation of chymotrypsinogen A and lysozyme
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.08 %
Cerium: ≤ 20 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 120 - 160 mg/ml
Functional test (b:a): ≤ 0.20
Functional test: Separation of chymotrypsinogen A and lysozyme
Legal Information
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1148940010 | 04022536575520 |
| 1148945000 | 04022536558868 |
| 1148940100 | 04022536741895 |
| 1148940500 | 04022536558851 |