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About This Item
NACRES:
NA.77
UNSPSC Code:
41106510
Quality Level
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
immunoblotting: suitable
application(s)
sample preparation
shipped in
dry ice
storage temp.
−70°C
General description
PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).
Features and Benefits
- Phosphorylation state preserved during extraction
- Compatible with kinase assays
Other Notes
Trim, J.E. and Sawyer, D.L. 2001. inNovations12, 5.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Toxic (F)
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
蛋白磷酸化是一种可逆的翻译后加工,在细胞内信号传递中有非常重要的功能。采用Western Blotting技术检测磷酸化蛋白是探讨信号传导中上游调控、下游功能调整、信息交流及反馈机制等的基本手段。我们提供专用的优质试剂和研究方法,帮助你获得准确、灵敏的磷酸化检测数据。
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 71296-3 | 07790788054229 |
| 71296-4 | 04055977254006 |