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Merck
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PCHT24H48

Millicell® 24 well hanging cell culture inserts

pore size 0.4 μm, clear PET membrane, diam. 6.5 mm, Tissue Culture (TC)-treated surface, sterile

Synonym(s):

permeable inserts, tissue culture inserts, transparent membrane insert, imaging insert

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About This Item

NACRES:
NB.13
UNSPSC Code:
41122107
Material:
clear PET membrane, polystyrene housing
Pore size:
0.4 μm
Sterility:
γ-irradiated, sterile
Binding type:
Tissue Culture (TC)-treated surface
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Quality Level

material

clear PET membrane, polystyrene housing

sterility

γ-irradiated, sterile

feature

4x106 pores/cm2

packaging

pack of 48 ea (individually blister packaged)

manufacturer/tradename

Millicell®

parameter

max. temp. at 37 °C

technique(s)

cell attachment: suitable, cell differentiation: suitable, drug transporter assay: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, permeability assay: suitable

H

15.5 mm

diam.

6.5 mm

insert maximum volume

0.4 mL

insert working volume

0.2 mL

membrane thickness

9-16 μm

size

24 wells

surface area

0.3 cm2

color

clear membrane, transparent membrane

pore size

0.4 μm

binding type

Tissue Culture (TC)-treated surface

General description

Millicell® cell culture inserts with polyethylene terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types.

This individually sterile-packaged insert has an optically clear 0.4 μm pore size membrane and fits in most 24-well tissue culture plates.

Application

  • Barrier Assays
  • Cell Proliferation
  • Cell Surface Receptors
  • Co-Culture
  • Epithelial Cell Growth
  • Feeder Layers
  • Fluorescent Detection /Immunohistochemistry
  • In Vitro Fertilization
  • In Vitro Toxicology
  • Microbial Attachment
  • Phase Contrast Microscopy
  • Polarized Protein Secretions
  • Polarized Uptake
  • Transport/Permeability

Features and Benefits

  • Optically clear TC-treated PET membrane
  • Low pore density (4 million pores/cm2)
  • Compatible with most 24-well plates
  • Apical assist rim to prevent membrane puncture or disruption of cells
  • Easily access the basolateral side between the flanges

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany


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Articles

Discover tips for choosing the best plate to use with Millicell® hanging inserts for your applications, including barrier formation and cell imaging.

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

Cell validation data using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.

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