PTHT12H48
Millicell® 12 well hanging cell culture inserts
pore size 0.4 μm, translucent PET membrane, diam. 12 mm, Tissue Culture (TC)-treated surface, sterile
Synonym(s):
Polyethylene Terephthalate hanging cell culture insert, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate
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About This Item
Quality Level
material
polystyrene housing, translucent PET membrane
sterility
γ-irradiated, sterile
feature
100x106 pores/cm2
packaging
pack of 48 ea (individually blister packaged)
manufacturer/tradename
Millicell®
parameter
37 °C max. temp.
technique(s)
cell attachment: suitable, cell differentiation: suitable, permeability assay: suitable
H
15.5 mm
diam.
12 mm
insert maximum volume
0.8 mL
insert working volume
0.4 mL
size
12 wells
surface area
1.1 cm2
color
translucent membrane
pore size
0.4 μm
binding type
Tissue Culture (TC)-treated surface
General description
In plastic tissue culture plates, cells can access media only from their apical sides, but in Millicell® tissue culture inserts, cells can access media from both their apical and basolateral sides. As a result, cell growth, structure, and function more closely mimic what occurs in vivo.
The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.
Application
- Cell Proliferation
- Cell Surface Receptors
- Co-Culture
- Epithelial Cell Growth
- Feeder Layers
- Fluorescent Detection / Immunohistochemistry
- In Vitro Toxicology
- Microbial Attachment
- Polarized Protein Secretions
- Polarized Uptake
- Transport / Permeability
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Protocols
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
Articles
How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MCHT12H48 | 04055977170252 |