Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Empirical Formula (Hill Notation):
C8H6BrClNO4P · C7H9N
CAS Number:
Molecular Weight:
433.62
UNSPSC Code:
12352204
PubChem Substance ID:
MDL number:
NACRES:
NA.54
description
5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt
assay
99% (HPLC)
form
powder
mol wt
Mr 326.44 (BCIP), Mr 433.6 (BCIP toluidine salt)
packaging
pkg of 1 g (11585002001), pkg of 250 mg (10760994001)
manufacturer/tradename
Roche
solubility
DMF: soluble
storage temp.
2-8°C
SMILES string
Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI
1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key
QEIFSLUFHRCVQL-UHFFFAOYSA-N
General description
BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
Analysis Note
Performance test: incubation with AP
Absorption: Absorption maximum in the visible light is not determined.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
Hazard Classifications
Acute Tox. 4 Dermal - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
136.4 °F
flash_point_c
58 °C
hcodes
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
David G Simmons et al.
Developmental biology, 304(2), 567-578 (2007-02-10)
Trophoblast giant cells (TGCs) are the first terminally differentiated subtype to form in the trophoblast cell lineage in rodents. In addition to mediating implantation, they are the main endocrine cells of the placenta, producing several hormones which regulate the maternal
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10760994001 | 04061838675408 |
| 11585002001 | 04061838251077 |