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About This Item
UNSPSC Code:
41105500
NACRES:
NA.54
Assay:
85% (HPLC)
Form:
solution
Storage temp.:
−20°C
assay
85% (HPLC)
form
solution
packaging
pkg of 125 μL (11573179910 [1 mM]]]), pkg of 25 μL (11573152910 [1 mM])
manufacturer/tradename
Roche
storage temp.
−20°C
General description
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP), alkali-labile is provided as in 1 mM tetralithium salt, solution. In which DIG is bound to dUTP via an alkali-sensitive ester linkage.
Application
Digoxigenin-11-dUTP, alkali-labile can be used for non-radioactive DNA labeling e.g. random primed or nick translation. It can also be used for labeling probes which are preferred in hybridization experiments where stripping and reprobing of the membrane is intended.
Biochem/physiol Actions
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphophate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.
Other Notes
For life science research only. Not for use in diagnostic procedures.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 11573152910 | 04061838703545 |
| 11573179910 | 04061838703552 |