Key Documents

View All Documentation

16658

X-GalNAc

Name: X-GalNAc
Brand: SIAL

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-4-chloro-3-indolyl 2-acetamido-2-deoxy-β-D-galactopyranoside, 5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide, X-Galactosaminide

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₈BrClN₂O₆

CAS Number:

129572-48-1

Molecular Weight:

449.68

UNSPSC Code:

12352200

PubChem Substance ID:

329751315

MDL number:

MFCD00083260

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

grade

Molecular Biology

sterility

non-sterile

assay

≥98.0% (TLC)

form

powder

mp

≥220 °C (lit.)

solubility

DMF: soluble, DMSO: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

SMILES string

CC(=O)N[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C16H18BrClN2O6/c1-6(22)20-13-15(24)14(23)10(5-21)26-16(13)25-9-4-19-8-3-2-7(17)12(18)11(8)9/h2-4,10,13-16,19,21,23-24H,5H2,1H3,(H,20,22)/t10-,13-,14+,15-,16-/m1/s1

InChI key

SUWPNTKTZYIFQT-LMXXTMHSSA-N

Description

General description

5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide (X-GalNAc)is a chromogenic substrate for for N-acetyl-β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates.

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for the detection of Candida albicans.

Biochem/physiol Actions

When X-GalNAc is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GalNAc turn blue in color, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

新产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Global Trade Item Number

SKU	GTIN
Y0001289	04061833806944
M3445-10MG	04061834050230