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About This Item
Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
Molecular Weight:
238.30
UNSPSC Code:
12352201
NACRES:
NA.31
PubChem Substance ID:
EC Number:
206-703-0
Beilstein/REAXYS Number:
4631
MDL number:
description
non-ionic
assay
≥99% (TLC)
form
powder
technique(s)
thin layer chromatography (TLC): suitable
impurities
10.0-22.0% Dioxane
color
white to off-white
mp
120 -122 °C ((248 - 252 °F ))
solubility
water: 50 mg/mL, clear, colorless
storage temp.
2-8°C
SMILES string
CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
Application
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Preparation Note
A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.
Other Notes
Non-metabolizable galactose analog.
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signalword
WarningDanger
Hazard Classifications
Carc. 1B - Eye Irrit. 2
supp_hazards
Storage Class
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| I5502-1G | 04061838446190 |
| I5502-10G | 04061838446183 |
| I5502-5G | 04061838446206 |